Inbred maize line PH86T

ABSTRACT

An inbred maize line, designated PH86T, the seeds and plants of inbred maize line PH86T, methods for producing a maize plant, either inbred or hybrid, produced by crossing the inbred maize line PH86T with another maize plant, and seed and plants produced therefrom. The invention also relates to methods for producing a modified PH86T maize plant that comprises in its genetic material one or more transgenes or backcross conversion genes and to the transgenic and backcross conversion maize plants produced by these methods. This invention also relates to methods for producing other inbred and hybrid maize lines derived from inbred maize line PH86T and to the inbred and hybrid maize lines so produced.

FIELD OF THE INVENTION

This invention relates generally to the field of maize breeding, specifically relating to an inbred maize line designated PH86T.

BACKGROUND OF THE INVENTION

The goal of plant breeding is to combine, in a single variety or hybrid, various desirable traits. For field crops, these traits may include resistance to diseases and insects, tolerance to heat and drought, reducing the time to crop maturity, greater yield, and better agronomic quality. With mechanical harvesting of many crops, uniformity of plant characteristics such as germination and stand establishment, growth rate, maturity, and plant and ear height, is important. Traditional plant breeding is an important tool in developing new and improved commercial crops.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred maize line, designated PH86T. This invention thus relates to the seeds of inbred maize line PH86T, to the plants of inbred maize line PH86T, to plant parts of inbred maize line PH86T, to methods for producing a maize plant produced by crossing the inbred maize line PH86T with another maize plant, including a plant that is part of a synthetic or natural population, and to methods for producing a maize plant containing in its genetic material one or more backcross conversion trait or transgene and to the backcross conversion maize plants and plant parts produced by those methods. This invention also relates to inbred maize lines and plant parts derived from inbred maize line PH86T, to methods for producing other inbred maize lines derived from inbred maize line PH86T and to the inbred maize lines and their parts derived by the use of those methods. This invention further relates to hybrid maize seeds, plants and plant parts produced by crossing the inbred line PH86T or a backcross conversion of PH86T with another maize line.

Definitions

Certain definitions used in the specification are provided below. Also in the examples that follow, a number of terms are used herein. In order to provide a clear and consistent understanding of the specification and claims, including the scope to be given such terms, the following definitions are provided. NOTE: ABS is in absolute terms and % MN is percent of the mean for the experiments in which the inbred or hybrid was grown. PCT designates that the trait is calculated as a percentage. % NOT designates the percentage of plants that did not exhibit a trait. For example, STKLDG % NOT is the percentage of plants in a plot that were not stalk lodged. These designators will follow the descriptors to denote how the values are to be interpreted.

ABTSTK=ARTIFICIAL BRITTLE STALK. A count of the number of “snapped” plants per plot following machine snapping. A snapped plant has its stalk completely snapped at a node between the base of the plant and the node above the ear. Expressed as percent of plants that did not snap.

ALLELE. Any of one or more alternative forms of a genetic sequence. Typically, in a diploid cell or organism, the two alleles of a given sequence occupy corresponding loci on a pair of homologous chromosomes.

ANT ROT=ANTHRACNOSE STALK ROT (Colletotrichum graminicola). A 1 to 9 visual rating indicating the resistance to Anthracnose Stalk Rot. A higher score indicates a higher resistance.

BACKCROSSING. Process in which a breeder crosses a progeny line back to one of the parental genotypes one or more times.

BARPLT=BARREN PLANTS. The percent of plants per plot that were not barren (lack ears).

BREEDING. The genetic manipulation of living organisms.

BREEDING CROSS. A cross to introduce new genetic material into a plant for the development of a new variety. For example, one could cross plant A with plant B, wherein plant B would be genetically different from plant A. After the breeding cross, the resulting F1 plants could then be selfed or sibbed for one, two, three or more times (F1, F2, F3, etc.) until a new inbred variety is developed. For clarification, such new inbred varieties would be within a pedigree distance of one breeding cross of plants A and B.

BRTSTK=BRITTLE STALKS. This is a measure of the stalk breakage near the time of pollination, and is an indication of whether a hybrid or inbred would snap or break near the time of flowering under severe winds. Data are presented as percentage of plants that did not snap.

CLDTST=COLD TEST. The percent of plants that germinate under cold test conditions.

CLN=CORN LETHAL NECROSIS. Synergistic interaction of maize chlorotic mottle virus (MCMV) in combination with either maize dwarf mosaic virus (MDMV-A or MDMV-B) or wheat streak mosaic virus (WSMV). A 1 to 9 visual rating indicating the resistance to Corn Lethal Necrosis. A higher score indicates a higher resistance.

COMRST=COMMON RUST (Puccinia sorghi). A 1 to 9 visual rating indicating the resistance to Common Rust. A higher score indicates a higher resistance.

D/D=DRYDOWN. This represents the relative rate at which a hybrid will reach acceptable harvest moisture compared to other hybrids on a 1 to 9 rating scale. A high score indicates a hybrid that dries relatively fast while a low score indicates a hybrid that dries slowly.

DIPERS=DIPLODIA EAR MOLD SCORES (Diplodia maydis and Diplodia macrospora). A 1 to 9 visual rating indicating the resistance to Diplodia Ear Mold. A higher score indicates a higher resistance.

DIPROT=DIPLODIA STALK ROT SCORE. Score of stalk rot severity due to Diplodia (Diplodia maydis). Expressed as a 1 to 9 score with 9 being highly resistant.

DRPEAR =DROPPED EARS. A measure of the number of dropped ears per lot and represents the percentage of plants that did not drop ears prior to harvest.

D/T=DROUGHT TOLERANCE. This represents a 1 to 9 rating for drought tolerance, and is based on data obtained under stress conditions. A high score indicates good drought tolerance and a low score indicates poor drought tolerance.

EARHT=EAR HEIGHT. The ear height is a measure from the ground to the highest placed developed ear node attachment and is measured in centimeters.

EARMLD=GENERAL EAR MOLD. Visual rating (1 to 9 score) where a “1” is very susceptible and a “9” is very resistant. This is based on overall rating for ear mold of mature ears without determining the specific mold organism, and may not be predictive for a specific ear mold.

EARSZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher the rating the larger the ear size.

EBTSTK=EARLY BRITTLE STALK. A count of the number of “snapped” plants per plot following severe winds when the corn plant is experiencing very rapid vegetative growth in the V5-V8 stage. Expressed as percent of plants that did not snap.

ECB1LF=EUROPEAN CORN BORER FIRST GENERATION LEAF FEEDING (Ostrinia nubilalis). A 1 to 9 visual rating indicating the resistance to preflowering leaf feeding by first generation European Corn Borer. A higher score indicates a higher resistance.

ECB2IT=EUROPEAN CORN BORER SECOND GENERATION INCHES OF TUNNELING (Ostrinia nubilalis). Average inches of tunneling per plant in the stalk.

ECB2SC=EUROPEAN CORN BORER SECOND GENERATION (Ostrinia nubilalis). A 1 to 9 visual rating indicating post flowering degree of stalk breakage and other evidence of feeding by European Corn Borer, Second Generation. A higher score indicates a higher resistance.

ECBDPE=EUROPEAN CORN BORER DROPPED EARS (Ostrinia nubilalis). Dropped ears due to European Corn Borer. Percentage of plants that did not drop ears under second generation corn borer infestation.

EGRWTH=EARLY GROWTH. This is a measure of the relative height and size of a corn seedling at the 2-4 leaf stage of growth. This is a visual rating (1 to 9), with 1 being weak or slow growth, 5 being average growth and 9 being strong growth. Taller plants, wider leaves, more green mass and darker color constitute higher score.

ELITE INBRED. An inbred that contributed desirable qualities when used to produce commercial hybrids. An elite inbred may also be used in further breeding for the purpose of developing further improved varieties.

ERTLDG=EARLY ROOT LODGING. Early root lodging is the percentage of plants that do not root lodge prior to or around anthesis; plants that lean from the vertical axis at an approximately 30° angle or greater would be counted as root lodged.

ERTLPN=EARLY ROOT LODGING. An estimate of the percentage of plants that do not root lodge prior to or around anthesis; plants that lean from the vertical axis at an approximately 30° angle or greater would be considered as root lodged.

ERTLSC=EARLY ROOT LODGING SCORE. Score for severity of plants that lean from a vertical axis at an approximate 30 degree angle or greater which typically results from strong winds prior to or around flowering recorded within 2 weeks of a wind event. Expressed as a 1 to 9 score with 9 being no lodging.

ESTCNT=EARLY STAND COUNT. This is a measure of the stand establishment in the spring and represents the number of plants that emerge on per plot basis for the inbred or hybrid.

EYESPT=EYE SPOT (Kabatiella zeae or Aureobasidium zeae). A 1 to 9 visual rating indicating the resistance to Eye Spot. A higher score indicates a higher resistance.

FUSERS=FUSARIUM EAR ROT SCORE (Fusarium moniliforme or Fusarium subglutinans). A 1 to 9 visual rating indicating the resistance to Fusarium ear rot. A higher score indicates a higher resistance.

GDU=GROWING DEGREE UNITS. Using the Barger Heat Unit Theory, which assumes that maize growth occurs in the temperature range 50° F. -86° F. and that temperatures outside this range slow down growth; the maximum daily heat unit accumulation is 36 and the minimum daily heat unit accumulation is 0. The seasonal accumulation of GDU is a major factor in determining maturity zones.

GDUSHD=GDU TO SHED. The number of growing degree units (GDUs) or heat units required for an inbred line or hybrid to have approximately 50 percent of the plants shedding pollen and is measured from the time of planting. Growing degree units are calculated by the Barger Method, where the heat units for a 24-hour period are: ${GDU} = {\frac{\left( {{Max}.\quad{temp}.{+ {{Min}.\quad{temp}.}}} \right)}{2} - 50}$

The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDUs to reach various stages of plant development.

GDUSLK=GDU TO SILK. The number of growing degree units required for an inbred line or hybrid to have approximately 50 percent of the plants with silk emergence from time of planting. Growing degree units are calculated by the Barger Method as given in GDU SHD definition.

GENOTYPE. Refers to the genetic constitution of a cell or organism.

GIBERS=GIBBERELLA EAR ROT (PINK MOLD) (Gibberella zeae). A 1 to 9 visual rating indicating the resistance to Gibberella Ear Rot. A higher score indicates a higher resistance.

GIBROT=GIBBERELLA STALK ROT SCORE. Score of stalk rot severity due to Gibberella (Gibberella zeae). Expressed as a 1 to 9 score with 9 being highly resistant.

GLFSPT=GRAY LEAF SPOT (Cercospora zeae-maydis). A 1 to 9 visual rating indicating the resistance to Gray Leaf Spot. A higher score indicates a higher resistance.

GOSWLT=GOSS'WILT (Corynepbacterium nebraskense). A 1 to 9 visual rating indicating the resistance to Goss'Wilt. A higher score indicates a higher resistance.

GRNAPP=GRAIN APPEARANCE. This is a 1 to 9 rating for the general appearance of the shelled grain as it is harvested based on such factors as the color of harvested grain, any mold on the grain, and any cracked grain. High scores indicate good grain quality.

HCBLT=HELMINTHOSPORIUM CARBONUM LEAF BLIGHT (Helminthosporium carbonum). A 1 to 9 visual rating indicating the resistance to Helminthosporium infection. A higher score indicates a higher resistance.

HD SMT=HEAD SMUT (Sphacelotheca reiliana). This score indicates the percentage of plants not infected.

HSKCVR=HUSK COVER. A 1 to 9 score based on performance relative to key checks, with a score of 1 indicating very short husks, tip of ear and kernels showing; 5 is intermediate coverage of the ear under most conditions, sometimes with thin husk; and a 9 has husks extending and closed beyond the tip of the ear. Scoring can best be done near physiological maturity stage or any time during dry down until harvested.

INC D/A=GROSS INCOME (DOLLARS PER ACRE). Relative income per acre assuming drying costs of two cents per point above 15.5 percent harvest moisture and current market price per bushel.

INCOME/ACRE. Income advantage of hybrid to be patented over other hybrid on per acre basis.

INC ADV=GROSS INCOME ADVANTAGE. GROSS INCOME advantage of variety #1 over variety #2.

KSZDCD=KERNEL SIZE DISCARD. The percent of discard seed; calculated as the sum of discarded tip kernels and extra large kernels.

LINKAGE. Refers to a phenomenon wherein alleles on the same chromosome tend to segregate together more often than expected by chance if their transmission was independent.

LINKAGE DISEQUILIBRIUM. Refers to a phenomenon wherein alleles tend to remain together in linkage groups when segregating from parents to offspring, with a greater frequency than expected from their individual frequencies.

L/POP=YIELD AT LOW DENSITY. Yield ability at relatively low plant densities on a 1 to 9 relative system with a higher number indicating the hybrid responds well to low plant densities for yield relative to other hybrids. A 1, 5, and 9 would represent very poor, average, and very good yield response, respectively, to low plant density.

LRTLDG=LATE ROOT LODGING. Late root lodging is the percentage of plants that do not root lodge after anthesis through harvest; plants that lean from the vertical axis at an approximately 30° angle or greater would be counted as root lodged.

LRTLPN=LATE ROOT LODGING. Late root lodging is an estimate of the percentage of plants that do not root lodge after anthesis through harvest; plants that lean from the vertical axis at an approximately 30° angle or greater would be considered as root lodged.

LRTLSC=LATE ROOT LODGING SCORE. Score for severity of plants that lean from a vertical axis at an approximate 30 degree angle or greater which typically results from strong winds after flowering. Recorded prior to harvest when a root-lodging event has occurred. This lodging results in plants that are leaned or “lodged” over at the base of the plant and do not straighten or “goose-neck” back to a vertical position. Expressed as a 1 to 9 score with 9 being no lodging.

MDMCPX=MAIZE DWARF MOSAIC COMPLEX (MDMV=Maize Dwarf Mosaic Virus and MCDV=Maize Chlorotic Dwarf Virus). A 1 to 9 visual rating indicating the resistance to Maize Dwarf Mosaic Complex. A higher score indicates a higher resistance.

MST=HARVEST MOISTURE. The moisture is the actual percentage moisture of the grain at harvest.

MSTADV=MOISTURE ADVANTAGE. The moisture advantage of variety #1 over variety #2 as calculated by: MOISTURE of variety #2−MOISTURE of variety #1 =MOISTURE ADVANTAGE of variety #1.

NLFBLT=NORTHERN LEAF BLIGHT (Helminthosporium turcicum or Exserohilum turcicum). A 1 to 9 visual rating indicating the resistance to Northern Leaf Blight. A higher score indicates a higher resistance.

OILT=GRAIN OIL. Absolute value of oil content of the kernel as predicted by Near-infrared Transmittance and expressed as a percent of dry matter.

PEDIGREE DISTANCE. Relationship among generations based on their ancestral links as evidenced in pedigrees. May be measured by the distance of the pedigree from a given starting point in the ancestry.

PLANT. As used herein, the term “plant” includes reference to an immature or mature whole plant, including a plant that has been detasseled or from which seed or grain has been removed. Seed or embryo that will produce the plant is also considered to be the plant.

PLANT PARTS. As used herein, the term “plant parts” includes leaves, stems, roots, seed, grain, embryo, pollen, ovules, flowers, ears, cobs, husks, stalks, root tips, anthers, silk, tissue, cells and the like.

PLANT CELL. Plant cell, as used herein includes, plant cells whether isolated, in tissue culture or incorporated in a plant or plant part.

PLTHT=PLANT HEIGHT. This is a measure of the height of the plant from the ground to the tip of the tassel in centimeters.

POLSC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount of pollen shed. The higher the score the more pollen shed.

POLWT=POLLEN WEIGHT. This is calculated by dry weight of tassels is collected as shedding commences minus dry weight from similar tassels harvested after shedding is complete.

POP K/A=PLANT POPULATIONS. Measured as 1000s per acre.

POP ADV=PLANT POPULATION ADVANTAGE. The plant population advantage of variety #1 over variety #2 as calculated by PLANT POPULATION of variety #2−PLANT POPULATION of variety #1=PLANT POPULATION ADVANTAGE of variety #1.

PRM=PREDICTED RELATIVE MATURITY. This trait, predicted relative maturity, is based on the harvest moisture of the grain. The relative maturity rating is based on a known set of checks and utilizes standard linear regression analyses and is also referred to as the Comparative Relative Maturity Rating System that is similar to the Minnesota Relative Maturity Rating System.

PRMSHD=A relative measure of the growing degree units (GDU) required to reach 50% pollen shed. Relative values are predicted values from the linear regression of observed GDU's on relative maturity of commercial checks.

PROT=GRAIN PROTEIN. Absolute value of protein content of the kernel as predicted by Near-Infrared Transmittance and expressed as a percent of dry matter.

RTLDG=ROOT LODGING. Root lodging is the percentage of plants that do not root lodge; plants that lean from the vertical axis at an approximately 30° angle or greater would be counted as root lodged.

RTLADV=ROOT LODGING ADVANTAGE. The root lodging advantage of variety #1 over variety #2.

SCTGRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount of scatter grain (lack of pollination or kernel abortion) on the ear. The higher the score the less scatter grain.

SDGVGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amount of vegetative growth after emergence at the seedling stage (approximately five leaves). A higher score indicates better vigor.

SEL IND=SELECTION INDEX. The selection index gives a single measure of the hybrid's worth based on information for up to five traits. A maize breeder may utilize his or her own set of traits for the selection index. One of the traits that is almost always included is yield. The selection index data presented in the tables represent the mean value averaged across testing stations.

SLFBLT=SOUTHERN LEAF BLIGHT (Heiminthosporium maydis or Bipolaris. maydis). A 1 to 9 visual rating indicating the resistance to Southern Leaf Blight. A higher score indicates a higher resistance.

SOURST=SOUTHERN RUST (Puccinia polysora). A 1 to 9 visual rating indicating the resistance to Southern Rust. A higher score indicates a higher resistance.

STAGRN=STAY GREEN. Stay green is the measure of plant health near the time of black layer formation (physiological maturity). A high score indicates better late-season plant health.

STDADV=STALK STANDING ADVANTAGE. The advantage of variety #1 over variety #2 for the trait STK CNT.

STKCNT=NUMBER OF PLANTS. This is the final stand or number of plants per plot.

STKLDG=STALK LODGING REGULAR. This is the percentage of plants that did not stalk lodge (stalk breakage) at regular harvest (when grain moisture is between about 20 and 30%) as measured by either natural lodging or pushing the stalks and determining the percentage of plants that break below the ear.

STKLDL=LATE STALK LODGING. This is the percentage of plants that did not stalk lodge (stalk breakage) at or around late season harvest (when grain moisture is between about 15 and 18%) as measured by either natural lodging or pushing the stalks and determining the percentage of plants that break below the ear.

STKLDS=STALK LODGING SCORE. A plant is considered as stalk lodged if the stalk is broken or crimped between the ear and the ground. This can be caused by any or a combination of the following: strong winds late in the season, disease pressure within the stalks, ECB damage or genetically weak stalks. This trait should be taken just prior to or at harvest. Expressed on a 1 to 9 scale with 9 being no lodging.

STLLPN=LATE STALK LODGING. This is the percent of plants that did not stalk lodge (stalk breakage or crimping) at or around late season harvest (when grain moisture is below 20%) as measure by either natural lodging or pushing the stalks and determining the percentage of plants that break or crimp below the ear.

STLPCN=STALK LODGING REGULAR. This is an estimate of the percentage of plants that did not stalk lodge (stalk breakage) at regular harvest (when grain moisture is between about 20 and 30%) as measured by either natural lodging or pushing the stalks and determining the percentage of plants that break below the ear.

STRT=GRAIN STARCH. Absolute value of starch content of the kernel as predicted by Near-infrared Transmittance and expressed as a percent of dry matter.

STWWLT=Stewart's Wilt (Erwinia stewartii). A 1 to 9 visual rating indicating the resistance to Stewart's Wilt. A higher score indicates a higher resistance.

TASBLS=TASSEL BLAST. A 1 to 9 visual rating was used to measure the degree of blasting (necrosis due to heat stress) of the tassel at the time of flowering. A 1 would indicate a very high level of blasting at time of flowering, while a 9 would have no tassel blasting.

TASSZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate the relative size of the tassel. The higher the rating the larger the tassel.

TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams) just prior to pollen shed.

TEXEAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate the relative hardness (smoothness of crown) of mature grain. A 1 would be very soft (extreme dent) while a 9 would be very hard (flinty or very smooth crown).

TILLER=TILLERS. A count of the number of tillers per plot that could possibly shed pollen was taken. Data are given as a percentage of tillers: number of tillers per plot divided by number of plants per plot.

TSTWT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grain in pounds for a given volume (bushel).

TSWADV=TEST WEIGHT ADVANTAGE. The test weight advantage of variety #1 over variety #2.

WIN M %=PERCENT MOISTURE WINS.

WIN Y %=PERCENT YIELD WINS.

YIELD BU/A=YIELD (BUSHELS/ACRE). Yield of the grain at harvest in bushels per acre adjusted to 15% moisture.

YLDADV=YIELD ADVANTAGE. The yield advantage of variety #1 over variety #2 as calculated by: YIELD of variety #1−YIELD variety #2=yield advantage of variety #1.

YLDSC=YIELD SCORE. A 1 to 9 visual rating was used to give a relative rating for yield based on plot ear piles. The higher the rating the greater visual yield appearance.

Definitions for Area of Adaptability

When referring to area of adaptability, such term is used to describe the location with the environmental conditions that would be well suited for this maize line. Area of adaptability is based on a number of factors, for example: days to maturity, insect resistance, disease resistance, and drought resistance. Area of adaptability does not indicate that the maize line will grow in every location within the area of adaptability or that it will not grow outside the area.

-   Central Corn Belt: Iowa, Illinois, Indiana -   Drylands: non-irrigated areas of North Dakota, South Dakota,     Nebraska, Kansas, Colorado and Oklahoma -   Eastern U.S.: Ohio, Pennsylvania, Delaware, Maryland, Virginia, and     West Virginia -   North central U.S.: Minnesota and Wisconsin -   Northeast: Michigan, New York, Vermont, and Ontario and Quebec     Canada -   Northwest U.S.: North Dakota, South Dakota, Wyoming, Washington,     Oregon, Montana, Utah, and Idaho -   South central U.S.: Missouri, Tennessee, Kentucky, Arkansas -   Southeast U.S.: North Carolina, South Carolina, Georgia, Florida,     Alabama, Mississippi, and Louisiana -   Southwest U.S.: Texas, Oklahoma, New Mexico, Arizona -   Western U.S.: Nebraska, Kansas, Colorado, and California -   Maritime Europe: France, Germany, Belgium and Austria

DETAILED DESCRIPTION OF THE INVENTION AND FURTHER EMBODIMENTS

All tables discussed in the Detailed Description of the Invention and Further Embodiments section can be found at the end of the section.

Morphological and Physiological Characteristics of PH86T

Inbred maize line PH86T is a yellow, flint-dent maize inbred that is best suited to be used as a female in the production of the first generation F1 maize hybrids. Inbred maize line PH86T is best adapted to the Southcentral and Southeastern areas of the United States, and can be used to produce hybrids with approximately 118 maturity based on the Comparative Relative Maturity Rating System for harvest moisture of grain. Inbred maize line PH86T demonstrates above average Gray Leaf Spot, Northern Leaf Blight, Southern Leaf Blight, Fusarium ear rot, Gibberella ear rot, and Common rusts tolerances as an inbred per se. In hybrid combination, inbred PH86T demonstrates above average yields across a wide range of environments and consistently stable yields in below average environments. In hybrid combination, PH86T also demonstrates above average staygreen and husk cover.

The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1, found at the end of the section). The inbred has been self-pollinated and ear-rowed a sufficient number of generations with careful attention paid to uniformity of plant type to ensure the homozygosity and phenotypic stability necessary for use in commercial hybrid seed production. The line has been increased both by hand and in isolated fields with continued observation for uniformity. No variant traits have been observed or are expected in PH86T.

Inbred maize line PH86T, being substantially homozygous, can be reproduced by planting seeds of the line, growing the resulting maize plants under self-pollinating or sib-pollinating conditions with adequate isolation, and harvesting the resulting seed using techniques familiar to the agricultural arts.

Genetic Marker Profile

To select and develop superior inbred parental lines for producing hybrids, it is necessary to identify and select genetically unique individuals that occur in a segregating population. The segregating population is the result of a combination of crossover events plus the independent assortment of specific combinations of alleles at many gene loci that results in specific and unique genotypes. The genetic variation among individual progeny of a breeding cross allows for the identification of rare and valuable new genotypes. Once identified, it is possible to utilize routine and predictable breeding methods to develop progeny that retain the rare and valuable new genotypes developed by the initial breeder.

Even if the entire genotypes of the parents of the breeding cross were characterized and a desired genotype known, only a few if any individuals having the desired genotype may be found in a large segregating F2 population. It would be very unlikely that a breeder of ordinary skill in the art would be able to recreate the same line twice from the very same original parents as the breeder is unable to direct how the genomes combine or how they will interact with the environmental conditions. This unpredictability results in the expenditure of large amounts of research resources in the development of a superior new maize inbred line. Once such a line is developed its value to society is substantial since it is important to advance the germplasm base as a whole in order to maintain or improve traits such as yield, disease resistance, pest resistance and plant performance in extreme weather conditions. Backcross trait conversions are routinely used to add or modify one or a few traits of such a line and this further enhances its value and usefulness to society.

Phenotypic traits exhibited by PH86T can be used to characterize the genetic contribution of PH86T to progeny lines developed through the use of PH86T. Quantitative traits including, but not limited to, yield, maturity, stay green, root lodging, stalk lodging , and early growth are typically governed by multiple genes at multiple loci. PH86T progeny plants that retain the same degree of phenotypic expression of these quantitative traits as PH86T have received significant genotypic and phenotypic contribution from PH86T. This characterization is enhanced when such quantitative trait is not exhibited in non-PH86T breeding material used to developed the PH86T progeny.

In addition to phenotypic observations, a plant can also be identified by its genotype. The genotype of a plant can be characterized through a genetic marker profile, which can identify plants of the same variety or a related variety or be used to determine or validate a pedigree. Genetic marker profiles can be obtained by techniques such as Restriction Fragment Length Polymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which are also referred to as Microsatellites, and Single Nucleotide Polymorphisms (SNPs). For example, see Berry, Don, et al., “Assessing Probability of Ancestry Using Simple Sequence Repeat Profiles: Applications to Maize Hybrids and Inbreds”, Genetics, 2002, 161:813-824, which is incorporated by reference herein.

Particular markers used for these purposes are not limited to the set of markers disclosed herein, but are envisioned to include any type of marker and marker profile which provides a means of distinguishing varieties. In addition to being used for identification of Inbred Line PH86T, a hybrid produced through the use of PH86T, and the identification or verification of pedigree for progeny plants produced through the use of PH86T, the genetic marker profile is also useful in further breeding and in-developing backcross conversions.

Means of performing genetic marker profiles using SSR polymorphisms are well known in the art. SSRs are genetic markers based on polymorphisms in repeated nucleotide sequences, such as microsatellites. A marker system based on SSRs can be highly informative in linkage analysis relative to other marker systems in that multiple alleles may be present. Another advantage of this type of marker is that, through use of flanking primers, detection of SSRs can be achieved, for example, by the polymerase chain reaction (PCR), thereby eliminating the need for labor-intensive Southern hybridization. The PCR™ detection is done by use of two oligonucleotide primers flanking the polymorphic segment of repetitive DNA. Repeated cycles of heat denaturation of the DNA followed by annealing of the primers to their complementary sequences at low temperatures, and extension of the annealed primers with DNA polymerase, comprise the major part of the methodology.

Following amplification, markers can be scored by gel electrophoresis of the amplification products. Scoring of marker genotype is based on the size of the amplified fragment as measured by molecular weight (MW) rounded to the nearest integer. While variation in the primer used or in laboratory procedures can affect the reported molecular weight, relative values should remain constant regardless of the specific primer or laboratory used. When comparing lines it is preferable if all SSR profiles are performed in the same lab. The SSR analyses reported herein were conducted in-house at Pioneer Hi-Bred. An SSR service is available to the public on a contractual basis by Paragen (formerly Celera AgGen) in Research Triangle Park, N.C.

Primers used for the SSRs reported herein are publicly available and may be found in the Maize GDB using the World Wide Wed. prefix followed by maizegdb.org (maintained by the USDA Agricultural Research Service), in Sharopova et al. (Plant Mol. Biol. 48(5-6):468-481), Lee et al (Plant Mol. Biol. 48(5-6):453-461), or may be construction from sequences if reported herein. Some marker information may be available from Paragen.

Map information is provided by bin number as reported in the Maize GDB. The bin number digits to the left of decimal point represent the chromosome on which such marker is located, and the digits to the right of the decimal represent the location on such chromosome. Any bin numbers reported in parenthesis represent other bin locations for such marker that have been reported in the literature or on the Maize GDB. A bin number.xx designation indicates that the bin location on that chromosome is not known. Map positions are also available on the Maize GDB for a variety of different mapping populations.

The SSR profile of Inbred PH86T can be found in Table 2 found at the end of this section. The profile can be used to identify hybrids comprising PH86T as a parent, since such hybrids will comprise two sets of allele, one set of which will be the same alleles as PH86T. Because an inbred is essentially homozygous at all relevant loci, an inbred should, in almost all cases, have only one allele at each locus. In contrast, a genetic marker profile of a hybrid should be the sum of those parents, e.g., if one inbred parent had allele x at a particular locus, and the other inbred parent had allele y the hybrid is x.y (heterozygous) by inference. Subsequent generations of progeny produced by selection and breeding are expected to be of genotype x (homozygous), y (homozygous), or x.y (heterozygous) for that locus position. When the F1 plant is used to produce an inbred, the inbred should be either x or y for that allele.

Plants and plant parts substantially benefiting from the use of PH86T in their development such as PH86T comprising a backcross conversion, transgene, or genetic sterility factor, may be identified by having a molecular marker profile with a high percent identity to PH86T.

The SSR profile of PH86T also can be used to identify essentially derived varieties and other progeny lines developed from the use of PH86T, as well as cells and other plant parts thereof. Progeny plants and plant parts produced using PH86T may be identified by having a molecular marker profile of at least 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69, 70%, 71%, 72%, 73%, 74%, 75%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5% genetic contribution from inbred line PH86T.

Unique SSR Profiles

While determining the SSR genetic marker profile of the inbreds described supra, several unique SSR profile loci sets were or may be identified through further analysis which did not appear in either parent of such inbred.

Such unique SSR profiles may arise during the breeding process from recombination or mutation. A combination of several unique alleles provides a means of identifying an inbred, a hybrid produced from such inbred, and progeny produced from such inbred. Such progeny may be further characterized as being within a pedigree distance of PH86T, such as within 1, 2, 3, 4 or 5 or less breeding crosses to a maize plant other than PH86T or a plant that has PH86T as a parent or other progenitor. Further unique molecular profiles may be identified with other molecular tools such as SNPs and RFLPs.

Each genetic marker profile represents a novel grouping of alleles unique to an inbred. Each of the unique SSR profiles that may be found are referred to as a “loci set”.

Comparing PH86T to other Inbreds

A breeder uses various methods to help determine which plants should be selected from the segregating populations and ultimately which inbred lines will be used to develop hybrids for commercialization. In addition to the knowledge of the germplasm and other skills the breeder uses, a part of the selection process is dependent on experimental design coupled with the use of statistical analysis. Experimental design and statistical analysis are used to help determine which plants, which family of plants, and finally which inbred lines and hybrid combinations are significantly better or different for one or more traits of interest. Experimental design methods are used to assess error so that differences between two inbred lines or two hybrid lines can be more accurately determined. Statistical analysis includes the calculation of mean values, determination of the statistical significance of the sources of variation, and the calculation of the appropriate variance components. Either a five or a one percent significance level is customarily used to determine whether a difference that occurs for a given trait is real or due to the environment or experimental error. One of ordinary skill in the art of plant breeding would know how to evaluate the traits of two plant varieties to determine if there is no significant difference between the two traits expressed by those varieties. For example, see Fehr, Walt, Principles of Cultivar Development, p. 261-286 (1987) which is incorporated herein by reference. Mean trait values may be used to determine whether trait differences are significant, and preferably the traits are measured on plants grown under the same environmental conditions.

In Tables 3A-3E, data from traits and characteristics of inbred maize line PH86T per se are given and compared to other maize inbred lines.

Inbred by Tester comparisons are also used to evaluate two inbreds. Inbred by tester comparison results can be used to distinguish two inbred lines while also providing information about the combining ability of the inbreds used. Tables 4A and 4B compare PH86T to two other inbreds, when each inbred is crossed to the same tester lines.

Methods of Introducing a New Gene or Trait into PH86T.

Field crops are bred through techniques that take advantage of the plant's method of pollination. A plant is self-pollinated if pollen from one flower is transferred to the same or another flower of the same plant. A plant is sib-pollinated when individuals within the same family or line are used for pollination. A plant is cross-pollinated if the pollen comes from a flower on a different plant from a different family or line. The terms “cross-pollination” and “out-cross” as used herein do not include self-pollination or sib-pollination.

Maize (zea mays L.), often referred to as corn in the United States, can be bred by both self-pollination and cross-pollination techniques. Maize has separate male and female flowers on the same plant, located on the tassel and the ear, respectively. Natural or open pollination occurs in maize when wind blows pollen from the tassels to the silks that protrude from the tops of the ears.

Plants that have been self-pollinated and selected for type for many generations become homozygous at almost all gene loci and produce a uniform population of true breeding progeny. A cross between two different homozygous lines produces a uniform population of hybrid plants that may be heterozygous for many gene loci. A cross of two plants; each heterozygous at a number of gene loci, will produce a population of hybrid plants that differ genetically and will not be uniform.

One possible method of introducing a new gene or trait into PH86T is producing a backcross conversion of PH86T. A backcross conversion occurs when DNA sequences are introduced through traditional (non-transformation) breeding techniques, such as backcrossing (Hallauer et al, 1988). DNA sequences, whether naturally occurring or transgenes, may be introduced using these traditional breeding techniques. A backcross conversion may produce a plant with a trait conversion in at least one or more crosses, including at least 2 crosses, at least 3 crosses, at least 4 crosses, and the like. Molecular marker assisted breeding or selection may be utilized to reduce the number of backcrosses necessary to achieve the backcross conversion. The term backcross conversion is also referred to in the art as a single locus conversion. Reference is made to US 2002/0062506A1 for a detailed discussion of single locus conversions and traits that may be incorporated into PH86T through backcross conversion. Desired traits transferred through this process include, but are not limited to, waxy starch, grain color, nutritional enhancements, altered fatty acid profile, increased digestibility, low phytate, industrial enhancements, disease resistance, insect resistance, herbicide resistance and yield enhancements. The trait of interest is transferred from the donor parent to the recurrent parent, in this case, the maize plant disclosed herein. The backcross conversion may result from either the transfer of a dominant allele or a recessive allele. Selection of progeny containing the trait of interest is accomplished by direct selection for a trait associated with a dominant allele. Selection of progeny for a trait that is transferred via a recessive allele, such as the waxy starch characteristic, requires growing and selfing the first backcross generation to determine which plants carry the recessive alleles. Recessive traits may require additional progeny testing in successive backcross generations to determine the presence of the gene of interest. Along with selection for the trait of interest, progeny are selected for the phenotype of the recurrent parent. While occasionally additional polynucleotide sequences or genes are transferred along with the backcross conversion, this is an insubstantial change to the variety, and backcross conversion lines are easily developed without undue experimentation. Hallauer et al. (1998, page 472) states that “For single gene traits that are relatively easy to classify, the backcross method is effective and relatively easy to manage.” Poehlman et al. (1995, page 334) states that “A backcross-derived inbred line fits into the same hybrid combination as the recurrent parent inbred line and contributes the effect of the additional gene added through the backcross.”

The advent of new molecular biological techniques has allowed the isolation and characterization of genetic elements with specific functions, such as encoding specific protein products. Scientists in the field of plant biology developed a strong interest in engineering the genome of plants to contain and express foreign genetic elements, or additional, or modified versions of native or endogenous genetic elements in order to alter the traits of a plant in a specific manner. Any DNA sequences, whether from a different species or from the same species, that are inserted into the genome using transformation are referred to herein collectively as “transgenes”. A transformed plant may contain at least one transgene but could contain at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and/or no more than 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2. Over the last fifteen to twenty years several methods for producing transgenic plants have been developed, and the present invention, in particular embodiments, also relates to transformed versions of the claimed inbred maize line PH86T as well as hybrid combinations thereof.

Numerous methods for plant transformation have been developed, including biological and physical plant transformation protocols. See, for example, Miki et al., “Procedures for Introducing Foreign DNA into Plants” in Methods in Plant Molecular Biology and Biotechnology, Glick, B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 67-88 and Armstrong, “The First Decade of Maize Transformation: A Review and Future Perspective” (Maydica 44:101-109, 1999). In addition, expression vectors and in vitro culture methods for plant cell or tissue transformation and regeneration of plants are available. See, for example, Gruber et al., “Vectors for Plant Transformation” in Methods in Plant Molecular Biology and Biotechnology, Glick, B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 89-119.

The most prevalent types of plant transformation involve the construction of an expression vector. Such a vector comprises a DNA sequence that contains a gene under the control of or operatively linked to a regulatory element, for example a promoter. The vector may contain one or more genes and one or more regulatory elements.

A genetic trait which has been engineered into a particular maize plant using transformation techniques, could be moved into another line using traditional breeding techniques that are well known in the plant breeding arts. For example, a backcrossing approach is commonly used to move a transgene from a transformed maize plant to an elite inbred line, and the resulting progeny would then comprise the transgene(s). Also, if an inbred line was used for the transformation then the transgenic plants could be crossed to a different inbred in order to produce a transgenic hybrid maize plant. As used herein, “crossing” can refer to a simple X by Y cross, or the process of backcrossing, depending on the context.

Various genetic elements can be introduced into the plant genome using transformation. These elements include but are not limited to genes; coding sequences; inducible, constitutive, and tissue specific promoters; enhancing sequences; and signal and targeting sequences. See the traits, genes and transforming methods listed in U.S. Pat. No. 6,118,055, which are herein incorporated by reference.

With transgenic plants according to the present invention, a foreign protein can be produced in commercial quantities. Thus, techniques for the selection and propagation of transformed plants, which are well understood in the art, yield a plurality of transgenic plants which are harvested in a conventional manner, and a foreign protein then can be extracted from a tissue of interest or from total biomass. Protein extraction from plant biomass can be accomplished by known methods which are discussed, for example, by Heney and Orr, Anal. Biochem. 114: 92-6 (1981). In one embodiment, the biomass of interest is seed.

A genetic map can be generated, primarily via conventional Restriction Fragment Length Polymorphisms (RFLP), Polymerase Chain Reaction (PCR) analysis, and Simple Sequence Repeats (SSR) and Single Nucleotide Polymorphisms (SNP) which identifies the approximate chromosomal location of the integrated DNA molecule. For exemplary methodologies in this regard, see Glick and Thompson, METHODS IN PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY 269-284 (CRC Press, Boca Raton, 1993).

Wang et al. discuss “Large Scale Identification, Mapping and Genotyping of Single-Nucleotide Polymorphisms in the Human Genome”, Science, 280:1077-1082, 1998, and similar capabilities are becoming increasingly available for the corn genome. Map information concerning chromosomal location is useful for proprietary protection of a subject transgenic plant. If unauthorized propagation is undertaken and crosses made with other germplasm, the map of the integration region can be compared to similar maps for suspect plants, to determine if the latter have a common parentage with the subject plant. Map comparisons would involve hybridizations, RFLP, PCR, SSR and sequencing, all of which are conventional techniques. SNP's may also be used alone or in combination with other techniques.

Likewise, by means of the present invention, plants can be genetically engineered to express various phenotypes of agronomic interest. Through the transformation of maize the expression of genes can be modulated to enhance disease resistance, insect resistance, herbicide resistance, agronomic traits as well as grain quality traits. Transformation can also be used to insert DNA sequences which control or help control male-sterility. DNA sequences native to maize as well as non-native DNA sequences can be transformed into maize and used to modulate levels of native or non-native proteins. Anti-sense technology, various promoters, targeting sequences, enhancing sequences, and other DNA sequences can be inserted into the maize genome for the purpose of modulating the expression of proteins. Exemplary transgenes implicated in this regard include, but are not limited to, those categorized below.

1. Transgenes that Confer Resistance to Pests or Disease and that Encode:

(A) Plant disease resistance genes. Plant defenses are often activated by specific interaction between the product of a disease resistance gene (R) in the plant and the product of a corresponding avirulence (Avr) gene in the pathogen. A plant variety can be transformed with cloned resistance gene to engineer plants that are resistant to specific pathogen strains. See, for example Jones et al., Science 266: 789 (1994) (cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum); Martin et al., Science 262: 1432 (1993) (tomato Pto gene for resistance to Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinos et at, Cell 78: 1089.(1994) (Arabidopsis RSP2 gene for resistance to Pseudomonas syringae). A plant resistant to a disease is one that is more resistant to a pathogen as compared to the wild type plant.

(B) A Bacillus thuringiensis protein, a derivative thereof or a synthetic polypeptide modeled thereon. See, for example, Geiser et al., Gene 48: 109 (1986), who disclose the cloning and nucleotide sequence of a Bt δ-endotoxin gene. Moreover, DNA molecules encoding δ-endotoxin genes can be purchased from American Type Culture Collection (Rockville, Md.), for example, under ATCC Accession Nos. 40098, 67136, 31995 and 31998. Other examples of Bacillus thuringiensis transgenes being genetically engineered are given in the following patents and hereby are incorporated by reference for this purpose: U.S. Pat. Nos. 5,188,960; 5,689,052; 5,880,275; and WO 97/40162.

(C) A lectin. See, for example, the disclosure by Van Damme et al., Plant Molec. Biol. 24: 25 (1994), who disclose the nucleotide sequences of several Clivia miniata mannose-binding lectin genes.

(D) A vitamin-binding protein such as avidin. See PCT application US93/06487 the contents of which are hereby incorporated by reference for this purpose. The application teaches the use of avidin and avidin homologues as larvicides against insect pests.

(E) An enzyme inhibitor, for example, a protease inhibitor or an amylase inhibitor. See, for example, Abe et al., J. Biol. Chem. 262: 16793 (1987) (nucleotide sequence of rice cysteine proteinase inhibitor), Huub et a., Plant Molec. Biol. 21: 985 (1993) (nucleotide sequence of cDNA encoding tobacco proteinase inhibitor I), and Sumitani et al, Biosci. Biotech. Biochem. 57: 1243 (1993) (nucleotide sequence of Streptomyces nitrosporeus α-amylase inhibitor) and U.S. Pat. No. 5,494,813.

(F) An insect-specific hormone or pheromone such as an ecdysteroid and juvenile hormone, a variant thereof, a mimetic based thereon, or an antagonist or agonist thereof. See, for example, the disclosure by Hammock et al., Nature 344: 458 (1990), of baculovirus expression of cloned juvenile hormone esterase, an inactivator of juvenile hormone.

(G) An insect-specific peptide or neuropeptide which, upon expression, disrupts the physiology of the affected pest. For example, see the disclosures of Regan, J. Biol. Chem. 269: 9 (1994) (expression cloning yields DNA coding for insect diuretic hormone receptor), and Pratt et al., Biochem. Biophys. Res. Comm. 163: 1243 (1989) (an allostatin is identified in Diploptera puntata). See also U.S. Pat. No. 5,266,317 to Tomalski et al., who disclose genes encoding insect-specific, paralytic neurotoxins.

(H) An insect-specific venom produced in nature by a snake, a wasp, etc. For example, see Pang et al., Gene 116: 165 (1992), for disclosure of heterologous expression in plants of a gene coding for a scorpion insectotoxic peptide.

(I) An enzyme responsible for an hyperaccumulation of a monterpene, a sesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivative or another non-protein molecule with insecticidal activity.

(J) An enzyme involved in the modification, including the post-translational modification, of a biologically active molecule; for example, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme, a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, a phosphatase, a kinase, a phosphorylase, a polymerase, an elastase, a chitinase and a glucanase, whether natural or synthetic. See PCT application WO 93/02197 in the name of Scott et al., which discloses the nucleotide sequence of a callase gene. DNA molecules which contain chitinase-encoding sequences can be obtained, for example, from the ATCC under Accession Nos. 39637 and 67152. See also Kramer et al., Insect Biochem. Molec. Biol23: 691 (1993), who teach the nucleotide sequence of a cDNA encoding tobacco hookworm chitinase, and Kawalleck et al., Plant Molec. Biol 21: 673 (1993), who provide the nucleotide sequence of the parsley ubi4-2 polyubiquitin gene.

(K) A molecule that stimulates signal transduction. For example, see the disclosure by Botella et al., Plant Molec. Biol. 24: 757 (1994), of nucleotide sequences for mung bean calmodulin cDNA clones, and Griess et al., Plant Physiol. 104; 1467 (1994), who provide the nucleotide sequence of a maize calmodulin cDNA clone.

(L) A hydrophobic moment peptide. See PCT application WO95/16776 (disclosure of peptide derivatives of Tachyplesin which inhibit fungal plant pathogens) and PCT application WO95/18855 (teaches synthetic antimicrobial peptides that confer disease resistance), the respective contents of which are hereby incorporated by reference for this purpose.

(M) A membrane permease, a channel former or a channel blocker. For example, see the disclosure by Jaynes et al., Plant Sci. 89: 43 (1993), of heterologous expression of a cecropin-β lytic peptide analog to render transgenic tobacco plants resistant to Pseudomonas solanacearum.

(N) A viral-invasive protein or a complex toxin derived therefrom. For example, the accumulation of viral coat proteins in transformed plant cells imparts resistance to viral infection and/or disease development effected by the virus from which the coat protein gene is derived, as well as by related viruses. See Beachy et al., Ann. Rev. Phytopathol. 28: 451 (1990). Coat protein-mediated resistance has been conferred upon transformed plants against alfalfa mosaic virus, cucumber mosaic virus, tobacco streak virus, potato virus X, potato virus Y, tobacco etch virus, tobacco rattle virus and tobacco mosaic virus. Id.

(O) An insect-specific antibody or an immunotoxin derived therefrom. Thus, an antibody targeted to a critical metabolic function in the insect gut would inactivate an affected enzyme, killing the insect. Cf Taylor et al., Abstract #497, SEVENTH INT'L SYMPOSIUM ON MOLECULAR PLANT-MICROBE INTERACTIONS (Edinburgh, Scotland, 1994) (enzymatic inactivation in transgenic tobacco via production of single-chain antibody fragments).

(P) A virus-specific antibody. See, for example, Tavladoraki et al., Nature 366: 469 (1993), who show that transgenic plants expressing recombinant antibody genes are protected from virus attack.

(Q) A developmental-arrestive protein produced in nature by a pathogen or a parasite. Thus, fungal endo α-1,4-D-polygalacturonases facilitate fungal colonization and plant nutrient release by solubilizing plant cell wall homo-α-1,4-D-galacturonase. See Lamb et al., Bio/Technology 10: 1436 (1992). The cloning and characterization of a gene which encodes a bean endopolygalacturonase-inhibiting protein is described by Toubart et al., Plant J. 2: 367 (1992).

(R) A developmental-arrestive protein produced in nature by a plant. For example, Logemann et al., Bio/Technology 10: 305 (1992), have shown that transgenic plants expressing the barley ribosome-inactivating gene have an increased resistance to fungal disease.

(S) Genes involved in the Systemic Acquired Resistance (SAR) Response and/or the pathogenesis related genes. Briggs, S., Current Biology, 5(2) (1995).

(T) Antifungal genes (Cornelissen and Melchers, PI. Physiol. 101:709-712, (1993) and Parijs et al., Planta 183:258-264, (1991) and Bushnell et al., Can. J. of Plant Path. 20(2):137-149 (1998).

2. Transgenes that Confer Resistance to a Herbicide, for Example:

(A) A herbicide that inhibits the growing point or meristem, such as an imidazolinone or a sulfonylurea. Exemplary genes in this category code for mutant ALS and AHAS enzyme as described, for example, by Lee et al., EMBO J. 7: 1241 (1988), and Miki et al., Theor Appl. Genet. 80: 449 (1990), respectively. See also, U.S. Pat. Nos. 5,605,011; 5,013,659; 5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373; 5,331,107; 5,928,937; and 5,378,824; and international publication WO 96/33270, which are incorporated herein by reference for this purpose.

(B) Glyphosate (resistance imparted by mutant 5-enolpyruvl-3-phosphikimate synthase (EPSP) and aroA genes, respectively) and other phosphono compounds such as glufosinate (phosphinothricin acetyl transferase (PAT) and Streptomyces hygroscopicus phosphinothricin acetyl transferase (bar) genes), and pyridinoxy or phenoxy proprionic acids and cycloshexones (ACCase inhibitor-encoding genes). See, for example, U.S. Pat. No. 4,940,835 to Shah et al., which discloses the nucleotide sequence of a form of EPSPS which can confer glyphosate resistance. U.S. Pat. No. 5,627,061 to Barry et al. also describes genes encoding EPSPS enzymes. See also U.S. Pat. Nos. 6,248,876 B1; 6,040,497; 5,804,425; 5,633,435; 5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775; 6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re. 36,449; RE 37,287 E; and 5,491,288; and international publications WO 97104103; WO 97/04114; WO 00/66746; WO 01/166704; WO 00166747 and WO 00166748, which are incorporated herein by reference for this purpose. Glyphosate resistance is also imparted to plants that express a gene that encodes a glyphosate oxido-reductase enzyme as described more fully in U.S. is Pat. Nos. 5,776,760 and 5,463,175, which are incorporated herein by reference for this purpose. In addition glyphosate resistance can be imparted to plants by the over expression of genes encoding glyphosate N-acetyltransferase. See, for example, U.S. Application Ser. Nos. 60/244,385; 60/377,175 and 60/377,719. A DNA molecule encoding a mutant aroA gene can be obtained under ATCC accession No. 39256, and the nucleotide sequence of the mutant gene is disclosed in U.S. Pat. No. 4,769,061 to Comai. European patent application No. 0 333 033 to Kumada et al. and U.S. Pat. No. 4,975,374 to Goodman et al. disclose nucleotide sequences of glutamine synthetase genes which confer resistance to herbicides such as L—phosphinothricin. The nucleotide sequence of a phosphinothricin-acetyl-transferase gene is provided in European patent No. 0 242 246 and 0 242 236 to Leemans et al De Greef et al., Bio/Technology 7: 61 (1989), describe the production of transgenic plants that express chimeric bar genes coding for phosphinothricin acetyl transferase activity. See also, U.S. Pat. Nos. 5,969,213; 5,489,520; 5,550,318; 5,874,265; 5,919,675; 5,561,236; 5,648,477; 5,646,024; 6,177,616 B1; and 5,879,903, which are incorporated herein by reference for this purpose. Exemplary of genes conferring resistance to phenoxy proprionic acids and cycloshexones, such as sethoxydim and haloxyfop, are the Acc1-S1, Acc1-S2 and Acc1-S3 genes described by Marshall et al, Theor Appl. Genet. 83: 435 (1992).

(C) A herbicide that inhibits photosynthesis, such as a triazine (psbA and gs+genes) and a benzonitrile (nitrilase gene). Przibilla et al., Plant Cell 3: 169 (1991), describe the transformation of Chlamydomonas with plasmids encoding mutant psbA genes. Nucleotide sequences for nitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker, and DNA molecules containing these genes are available under ATCC Accession Nos. 53435, 67441 and 67442. Cloning and expression of DNA coding for a glutathione S-transferase is described by Hayes et al., Biochem. J. 285: 173 (1992).

(D) Acetohydroxy acid synthase, which has been found to make plants that express this enzyme resistant to multiple types of herbicides, has been introduced into a variety of plants (see, e.g., Hattori et al. (1995) Mol Gen Genet 246:419). Other genes that confer tolerance to herbicides include: a gene encoding a chimeric protein of rat cytochrome P4507A1 and yeast NADPH-cytochrome P450 oxidoreductase (Shiota et al. (1994) Plant PhysiolPlant Physiol 106:17), genes for glutathione reductase and superoxide dismutase (Aono et al. (1995) Plant Cell Physiol 36:1687, and genes for various phosphotransferases (Datta et al. (1992) Plant Mol Biol 20:619).

(E) Protoporphyrinogen oxidase (protox) is necessary for the production of chlorophyll, which is necessary for all plant survival. The protox enzyme serves as the target for a variety of herbicidal compounds. These herbicides also inhibit growth of all the different species of plants present, causing their total destruction. The development of plants containing altered protox activity which are resistant to these herbicides are described in U.S. Pat. Nos. 6,288,306 B1; 6,282,837 B1; and 5,767,373; and international publication WO 01/12825, which are incorporated herein by reference for this purpose.

3. Transgenes that Confer or Contribute to a Grain Trait, Such as:

(A) Modified fatty acid metabolism, for example, by transforming a plant with an antisense gene of stearoyl-ACP desaturase to increase stearic acid content of the plant. See Knultzon et al., Proc. Natl. Acad. Sci. USA 89: 2624 (1992).

(B) Decreased Phytate Content

-   -   (1) Introduction of a phytase-encoding gene would enhance         breakdown of phytate, adding more free phosphate to the         transformed plant. For example, see Van Hartingsveldt et al.,         Gene 127: 87 (1993), for a disclosure of the nucleotide sequence         of an Aspergillus niger phytase gene.     -   (2) A gene could be introduced that reduces phytate content. In         maize, this, for example, could be accomplished, by cloning and         then reintroducing DNA associated with the single allele which         is responsible for maize mutants characterized by low levels of         phytic acid. See Raboy et al., Maydica 35: 383 (1990).

(C) Modified carbohydrate composition effected, for example, by transforming plants with a gene coding for an enzyme that alters the branching pattern of starch. See Shiroza et al., J. Bacteriol. 170: 810 (1988) (nucleotide sequence of Streptococcus mutans fructosyltransferase gene), Steinmetz et al., Mol. Gen. Genet. 200: 220 (1985) (nucleotide sequence of Bacillus subtilis levansucrase gene), Pen et al, Bio/Technology 10: 292 (1992) (production of transgenic plants that express Bacillus licheniformis α-amylase), Elliot et al., Plant Molec. Biol. 21: 515 (1993) (nucleotide sequences of tomato invertase genes), Søgaard et al, J. Biol. Chem. 268: 22480 (1993) (site-directed mutagenesis of barley c-amylase gene), and Fisher et al., Plant Physiol. 102: 1045 (1993) (maize endosperm starch branching enzyme II).

(D) Elevated oleic acid via FAD-2 gene modification and/or decreased linolenic acid via FAD-3 gene modification (see U.S. Pat. No. 6,063,947; 6,323,392; and WO 93/111245).

4. Genes that Control Male-sterility

(A) Introduction of a deacetylase gene under the control of a tapetum-specific promoter and with the application of the chemical N-Ac-PPT (WO 01/29237).

(B) Introduction of various stamen-specific promoters (WO 92/13956, WO 92/13957).

(C) Introduction of the barnase and the barstar gene (Paul et al. Plant Mol. Biol. 19:611-622, 1992).

PH86T Inbreds that are Male Sterile

Hybrid seed production requires elimination or inactivation of pollen produced by the female inbred parent. Incomplete removal or inactivation of the pollen provides the potential for self-pollination. A reliable method of controlling male fertility in plants offers the opportunity for improved plant breeding. This is especially true for development of maize hybrids, which relies upon some sort of male sterility system. It should be understood that PH86T can be produced in a male-sterile form. There are several ways in which a maize plant can be manipulated so that it is male sterile. These include use of manual or mechanical emasculation (or detasseling), use of one or more genetic factors that confer male sterility, including cytoplasmic genetic and/or nuclear genetic male sterility, use of gametocides and the like. A male sterile inbred designated PH86T may include one or more genetic factors, which result in cytoplasmic genetic and/or nuclear genetic male sterility. Such embodiments are also within the scope of the present claims. The term manipulated to be male sterile refers to the use of any available techniques to produce a male sterile version of maize line PH86T. The male sterility may be either partial or complete male sterility.

Hybrid maize seed is often produced by a male sterility system incorporating manual or mechanical detasseling. Alternate strips of two maize inbreds are planted in a field, and the pollen-bearing tassels are removed from one of the inbreds (female). Provided that there is sufficient isolation from sources of foreign maize pollen, the ears of the detasseled inbred will be fertilized only from the other inbred (male), and the resulting seed is therefore hybrid and will form hybrid plants.

The laborious detasseling process can be avoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMS inbred are male sterile as a result of factors resulting from the cytoplasmic, as opposed to the nuclear, genome. Thus, this characteristic is inherited exclusively through the female parent in maize plants, since only the female provides cytoplasm to the fertilized seed. CMS plants are fertilized with pollen from another inbred that is not male-sterile. Pollen from the second inbred may or may not contribute genes that make the hybrid plants male-fertile, and either option may be preferred depending on the intended use of the hybrid. The same hybrid seed, a portion produced from detasseled fertile maize and a portion produced using the CMS system, can be blended to insure that adequate pollen loads are available for fertilization when the hybrid plants are grown.

There are several methods of conferring genetic male sterility available, such as multiple mutant genes at separate locations within the genome that confer male sterility, as disclosed in U.S. Pat. No. 4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations as described by Patterson in U.S. Pat. No. 3,861,709 and 3,710,511. In addition to these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. Pat. No. 5,432,068, have developed a system of nuclear male sterility which includes: identifying a gene which is critical to male fertility; silencing this native gene which is critical to male fertility; removing the native promoter from Is the essential male fertility gene and replacing it with an inducible promoter; inserting this genetically engineered gene back into the plant; and thus creating a plant that is male sterile because the inducible promoter is not “on” resulting in the male fertility gene not being transcribed. Fertility is restored by inducing, or turning “on” , the promoter, which in turn allows the gene that confers male fertility to be transcribed.

These, and the other methods of conferring genetic male sterility in the art, each possess their own benefits and drawbacks. Some other methods use a variety of approaches such as delivering into the plant a gene encoding a cytotoxic substance associated with a male tissue specific promoter or an antisense system in which a gene critical to fertility is identified and an antisense to that gene is inserted in the plant (see: Fabinjanski, et al. EPO 89/3010153.8 publication no. 329,308 and PCT application PCT/CA90/00037 published as WO 90/08828).

Another system for controlling male sterility makes use of gametocides. Gametocides are not a genetic system, but rather a topical application of chemicals. These chemicals affect cells that are critical to male fertility. The application of these chemicals affects fertility in the plants only for the growing season in which the gametocide is applied (see Carlson, Glenn R., U.S. Pat. No. 4,936,904). Application of the gametocide, timing of the application and genotype specificity often limit the usefulness of the approach and it is not appropriate in all situations.

Incomplete control over male fertility may result in self-pollinated seed being unintentionally harvested and packaged with hybrid seed. The male parent is grown next to the female parent in the field, so there is the very low probability that the male selfed seed could be unintentionally harvested and packaged with the hybrid seed. Once the seed from the hybrid bag is planted, it is possible to identify and select these self-pollinated plants. These self-pollinated plants will be genetically equivalent to one of the inbred lines used to produce the hybrid. Though the possibility of inbreds being included hybrid seed bags exists, the occurrence is very low because much care is taken to avoid such inclusions. It is worth noting that hybrid seed is sold to growers for the production of grain or forage and not for breeding or seed production.

These self-pollinated plants can be identified and selected by one skilled in the art due to their decreased vigor when compared to the hybrid. Inbreds are identified by their less vigorous appearance for vegetative and/or reproductive characteristics, including shorter plant height, small ear size, ear and kernel shape, cob color, or other characteristics.

Identification of these self-pollinated lines can also be accomplished through molecular marker analyses. See, “The Identification of Female Selfs in Hybrid Maize: A Comparison Using Electrophoresis and Morphology”, Smith, J. S. C. and Wych, R. D., Seed Science and Technology 14, pp. 1-8 (1995), the disclosure of which is expressly incorporated herein by reference. Through these technologies, the homozygosity of the self pollinated line can be verified by analyzing allelic composition at various loci along the genome. Those methods allow for rapid identification of the invention disclosed herein. See also, “Identification of Atypical Plants in Hybrid Maize Seed by Postcontrol and Electrophoresis” Sarca, V. et al., Probleme de Genetica Teoritica si Aplicata Vol. 20 (1) p. 29-42.

Development of Maize hybrids using PH86T

A single cross maize hybrid results from the cross of two inbred lines, each of which has a genotype that complements the genotype of the other. The hybrid progeny of the first generation is designated F1. In the development of commercial hybrids in a maize plant breeding program, only the F1 hybrid plants are sought. F1 hybrids are more vigorous than their inbred parents. This hybrid vigor, or heterosis, can be manifested in many polygenic traits, including increased vegetative growth and increased yield.

PH86T may be used to produce hybrid maize seed. One such embodiment is the method of crossing inbred maize line PH86T with another maize plant, such as a different maize inbred line, to form a first generation F1 hybrid plant. The first generation F1 hybrid plant produced by this method is also an embodiment of the invention. This first generation F1 plant will comprise an essentially complete set of the alleles of inbred line PH86T. One of ordinary skill in the art can utilize either breeder books or molecular methods to identify a particular F1 hybrid plant produced using inbred line PH86T, and any such individual plant is also encompassed by this invention. These embodiments also cover use of these methods with transgenic, male sterile and/or backcross conversions of inbred line PH86T.

The development of a maize hybrid in a maize plant breeding program involves three steps: (1) the selection of plants from various germplasm pools for initial breeding crosses; (2) the selfing of the selected plants from the breeding crosses for several generations to produce a series of inbred lines, which, although different from each other, breed true and are highly uniform; and (3) crossing the selected inbred lines with different inbred lines to produce the hybrids. During the inbreeding process in maize, the vigor of the lines decreases. Vigor is restored when two different inbred lines are crossed to produce the hybrid. An important consequence of the homozygosity and homogeneity of the inbred lines is that the hybrid between a defined pair of inbreds will always be the same. Once the inbreds that give a superior hybrid have been identified, the hybrid seed can be reproduced indefinitely as long as the homogeneity of the inbred parents is maintained.

PH86T may be used to produce a single cross hybrid, a three-way hybrid or a double cross hybrid. A single cross hybrid is produced when two inbred lines are crossed to produce the F1 progeny. A double cross hybrid is produced from four inbred lines crossed in pairs (A×B and C×D) and then the two F1 hybrids are crossed again (A×B)×(C×D). A three-way cross hybrid is produced from three inbred lines where two of the inbred lines are crossed (A×B) and then the resulting F1 hybrid is crossed with the third inbred (A×B)×C. Much of the hybrid vigor and uniformity exhibited by F1 hybrids is lost in the next generation (F2). Consequently seed produced from hybrids is not used for planting stock.

Inbred by Tester Comparisons

Combining ability of a line, as well as the performance of the line per se, is a factor in the selection of improved maize inbreds. Combining ability refers to a line's contribution as a parent when crossed with other lines to form hybrids. The hybrids formed for the purpose of selecting superior lines are designated as test crosses. One way of measuring combining ability is by using values based in part on the overall mean of a number of test crosses. This mean may be adjusted to remove environmental effects and it is adjusted for known genetic relationships among the lines.

Combining Ability Data

A general combining ability report for inbred PH86T is provided in Table 5. Table 5 shows overall mean values of traits from numerous F1 lines produced by crosses between inbred PH86T and other maize lines. Table 5 demonstrates that inbred PH86T shows good general combining ability for hybrid production.

A specific combining ability report for inbred PH86T is provided in Tables 5A and 5B. Tables 5A and 5B show mean values of traits from numerous individual F1 lines produced by crosses between inbred PH86T and other maize lines.

Hybrid Comparisons

The results in Tables 6A-6D compare a hybrid for which inbred PH86T is a parent and four other hybrids.

Using PH86T to Develop other Maize Inbreds

Inbred maize lines are typically developed for use in the production of hybrid maize lines. However, PH86T could also be used to derive new maize inbred lines. Inbred maize lines preferably should be highly homogeneous, substantially homozygous and reproducible to be useful as parents of commercial hybrids. Plant breeding techniques known in the art and used in a maize plant breeding program include, but are not limited to, recurrent selection, mass selection, bulk selection, mass selection, backcrossing, pedigree breeding, open pollination breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, making double haploids, and transformation. Often combinations of these techniques are used. The development of maize hybrids in a maize plant breeding program requires, in general, the development of homozygous inbred lines, the crossing of these lines, and the evaluation of the crosses. There are Is many analytical methods available to evaluate the result of a cross. The oldest and most traditional method of analysis is the observation of phenotypic traits. Alternatively, the genotype of a plant can be examined.

Using PH86T In a Breeding Program

This invention is directed to methods for producing a maize plant by crossing a first parent maize plant with a second parent maize plant wherein either the first or second parent maize plant is an inbred maize plant of the line PH86T. Thus, any such methods using the inbred maize line PH86T are part of this invention: selfing, sibbing, backcrosses, mass selection, bulk selection, hybrid production, crosses to populations, and the like. All plants produced using inbred maize line PH86T as a parent are within the scope of this invention, including plants essentially derived from inbred maize line PH86T, as such term is defined in 7 U.S.C. § 2104(a)(3) of the Plant Variety Protection Act, which definition is hereby incorporated by reference. This also includes progeny plants and parts thereof with at least one ancestor that is PH86T, and more specifically, where the pedigree of the progeny includes 1, 2, 3, 4, and/or 5 or less breeding crosses to a maize plant other than PH86T or a plant that has PH86T as a parent or other progenitor. All breeders of ordinary skill in the art maintain pedigree records of their breeding programs. These pedigree records contain a detailed description of the breeding process, including a listing of all parental lines used in the breeding process and information on how such line was used. Thus, a breeder would know if PH86T were used in the development of a progeny line, and would also know how many crosses to a line other than PH86T or line with PH86T as a parent or other progenitor were made in the development of any progeny line. The inbred maize line may also be used in crosses with other, different, maize inbreds to produce first generation (F1) maize hybrid seeds and plants with superior characteristics.

Pedigree Breeding

Pedigree breeding starts with the crossing of two genotypes, such as PH86T and one other elite inbred line having one or more desirable characteristics that is lacking or which complements PH86T. If the two original parents do not provide all the desired characteristics, other sources can be included in the breeding population. In the pedigree method, superior plants are selfed and selected in successive filial generations. In the succeeding filial generations the heterozygous condition gives way to homogeneous lines as a result of self-pollination and selection. Typically in the pedigree method of breeding, five or more successive filial generations of selfing and selection is practiced: F1→F2; F2→F3; F3 →F4; F4 →F₅, etc. After a sufficient amount of inbreeding, successive filial generations will serve to increase seed of the developed inbred. Preferably, an inbred line comprises homozygous alleles at about 95% or more of its loci.

Backcrossing

In addition to being used to create a backcross conversion, backcrossing can also be used to modify PH86T and a hybrid that is made using the modified PH86T. As discussed previously, backcrossing can be used to transfer a specific desirable trait from one line, the donor parent, to an inbred called the recurrent parent which has overall good agronomic characteristics yet lacks that desirable trait. This transfer of the desirable trait into an inbred with overall good agronomic characteristics can be accomplished by first crossing a recurrent parent and a donor parent (non-recurrent parent). The progeny of this cross is then mated back to the recurrent parent followed by selection in the resultant progeny for the desired trait to be transferred from the non-recurrent parent as well as selection for the characteristics of the recurrent parent. Typically after four or more backcross generations with selection for the desired trait and the characteristics of the recurrent parent, the progeny will contain essentially all genes of the recurrent parent except for the genes controlling the desired trait. However, the number of backcross generations can be less if molecular markers are used during selection or elite germplasm is used as the donor parent. The last backcross generation is then selfed to give pure breeding progeny for the gene(s) being transferred. In addition to the above described use, backcrossing can be used in conjunction with pedigree breeding to develop new inbred lines. For example, an F1 can be created that is backcrossed to one of its parent lines to create is a BC1, BC2, BC3, etc. Progeny are selfed and selected so that the newly developed inbred has many of the attributes of the recurrent parent and some of the desired attributes of the non-recurrent parent. This approach leverages the value and strengths of the recurrent parent for use in new hybrids and breeding, and has very significant value for a breeder.

Recurrent Selection and Mass Selection

Recurrent selection is a method used in a plant breeding program to improve a population of plants. PH86T can be used in a recurrent selection program to improve the population. The method entails individual plants cross pollinating with each other to form progeny which are then grown. The superior progeny are then selected by any number of methods, which include individual plant, half-sib progeny, full-sib progeny, selfed progeny and topcrossing. The selected progeny are cross pollinated with each other to form progeny for another population. This population is planted and again superior plants are selected to cross pollinate with each other. Recurrent selection is a cyclical process and therefore can be repeated as many times as desired. The objective of recurrent selection is to improve the traits of a population. The improved population can then be used as a source of breeding material to obtain inbred lines to be used in hybrids or used as parents for a synthetic cultivar. A synthetic cultivar is the resultant progeny formed by the intercrossing of several selected inbreds. Mass selection is a useful technique when used in conjunction with molecular marker enhanced selection. In mass selection seeds from individuals are selected based on phenotype. These selected seeds are then bulked and used to grow the next generation. Similarly, bulk selection requires growing a population of plants in a bulk plot, allowing the plants to self-pollinate, harvesting the seed in bulk and then using a sample of the seed harvested in bulk to plant the next generation. Also, instead of directed pollination, open pollination could be used as part of the breeding program.

Mutation Breeding

Mutation breeding is one of many methods that could be used to introduce new traits into PH86T. Mutations that occur spontaneously or are artificially induced can be useful sources of variability for a plant breeder. The goal of artificial mutagenesis is to increase the rate of mutation for a desired characteristic. Mutation rates can be increased by many different means including temperature, long-term seed storage, tissue culture conditions, radiation; such as X-rays, Gamma rays (e.g. cobalt 60 or cesium 137), neutrons, (product of nuclear fission by uranium 235 in an atomic reactor), Beta radiation (emitted from radioisotopes such as phosphorus 32 or carbon 14), or ultraviolet radiation (preferably from 2500 to 2900 nm), or chemical mutagens (such as base analogues (5-bromo-uracil), related compounds (8-ethoxy caffeine), antibiotics (streptonigrin), alkylating agents (sulfur mustards, nitrogen mustards, epoxides, ethylenamines, sulfates, sulfonates, sulfones, lactones), azide, hydroxylamine, nitrous acid, or acridines. Once a desired trait is observed through mutagenesis the trait may then be incorporated into existing germplasm by traditional breeding techniques. Details of mutation breeding can be found in “Principals of Cultivar Development” Fehr, 1993 Macmillan Publishing Company the disclosure of which is incorporated herein by reference.

Breeding with Molecular Markers

Molecular markers, which includes markers identified through the use of techniques such as Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) and Single Nucleotide Polymorphisms (SNPs), may be used in plant breeding methods utilizing PH86T.

Isozyme Electrophoresis and RFLPs as discussed in Lee, M., “Inbred Lines of Maize and Their Molecular Markers,” The Maize Handbook, (Springer-Verlag, New York, Inc. 1994, at 423-432) incorporated herein by reference for this purpose, have been widely used to determine genetic composition. Isozyme Electrophoresis has a relatively low number of available markers and a low number of allelic variants among maize inbreds. RFLPs allow more discrimination because they have a higher degree of allelic variation in maize and a larger number of markers can be found. Both of these methods have been eclipsed by SSRs as discussed in Smith et al., “An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree”, Theoretical and Applied Genetics (1997) vol. 95 at 163-173 and by Pejic et al., “Comparative analysis of genetic similarity among maize inbreds detected by RFLPs, RAPDs, SSRs, and AFLPs,” Theoretical and Applied Genetics (1998) at 1248-1255 incorporated herein by reference. SSR technology is more efficient and practical to use than RFLPs; more marker loci can be routinely used and more alleles per marker locus can be found using SSRs in comparison to RFLPs. Single Nucleotide Polymorphisms may also be used to identify the unique genetic composition of the invention and progeny lines retaining that unique genetic composition. Various molecular marker techniques may be used in combination to enhance overall resolution.

Maize DNA molecular marker linkage maps have been rapidly constructed and widely implemented in genetic studies. One such study is described in Boppenmaier, et al., “Comparisons among strains of inbreds for RFLPs”, Maize Genetics Cooperative Newsletter, 65:1991, pg. 90, is incorporated herein by reference.

One use of molecular markers is Quantitative Trait Loci (QTL) mapping. QTL mapping is the use of markers, which are known to be closely linked to alleles that have measurable effects on a quantitative trait. Selection in the breeding process is based upon the accumulation of markers linked to the positive effecting alleles and/or the elimination of the markers linked to the negative effecting alleles from the plant's genome.

Molecular markers can also be used during the breeding process for the selection of qualitative traits. For example, markers closely linked to alleles or markers containing sequences within the actual alleles of interest can be used to select plants that contain the alleles of interest during a backcrossing breeding program. The markers can also be used to select for the genome of the recurrent parent and against the genome of the donor parent. Using this procedure can is minimize the amount of genome from the donor parent that remains in the selected plants. It can also be used to reduce the number of crosses back to the recurrent parent needed in a backcrossing program. The use of molecular markers in the selection process is often called genetic marker enhanced selection.

Production of Double Haploids

The production of double haploids can also be used for the development of inbreds in the breeding program. An F1 hybrid for which PH86T is a parent can be used to produce double haploid plants. Double haploids are produced by the doubling of a set of chromosomes (1 N) from a heterozygous plant to produce a completely homozygous individual. For example, see Wan et al., “Efficient Production of Doubled Haploid Plants Through Colchicine Treatment of Anther-Derived Maize Callus”, Theoretical and Applied Genetics, 77:889-892, 1989. This can be advantageous because the process omits the generations of selfing needed to obtain a homozygous plant from a heterozygous source.

Use Of PH86T In Tissue Culture

This invention is also directed to the use of PH86T in tissue culture. As used herein, the term “tissue culture” includes plant protoplasts, plant cell tissue culture, cultured microspores, plant calli, plant clumps, and the like. As used herein, phrases such as “growing the seed” or “grown from the seed” include embryo rescue, isolation of cells from seed for use in tissue culture, as well as traditional growing methods.

Duncan, Williams, Zehr, and Widholm, Planta (1985) 165:322-332 reflects that 97% of the plants cultured that produced callus were capable of plant regeneration. Subsequent experiments with both inbreds and hybrids produced 91% regenerable callus that produced plants. In a further study in 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988), 7:262-265 reports several media additions that enhance regenerability of callus of two inbred lines. Other published reports also indicated that “nontraditional” tissues are capable of producing somatic embryogenesis and plant regeneration. K. P. Rao, et al., Maize Genetics Cooperation Newsletter, 60:64-65 (1986), refers to somatic embryogenesis from glume callus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345-347 (1987) indicates somatic embryogenesis from the tissue cultures of maize, leaf segments. Thus, it is clear from the literature that the state of the art is such that these methods of obtaining plants are, and were, “conventional” in the sense that they are routinely used and have a very high rate of success.

Tissue culture of maize, including tassel/anther culture, is described in U.S. 2002/10062506A1 and European Pat. Application, publication 160,390, each of which are incorporated herein by reference for this purpose. Maize tissue culture procedures are also described in Green and Rhodes, “Plant Regeneration in Tissue Culture of Maize,” Maize for Biological Research (Plant Molecular Biology Association, Charlottesville, Va. 1982, at 367-372) and in Duncan, et al., “The Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea Mays Genotypes,” 165 Planta 322-332 (1985). Thus, another aspect of this invention is to provide cells which upon growth and differentiation produce maize plants having the genotype and/or physiological and morphological characteristics of inbred line PH86T.

Progeny of the breeding methods described herein may be characterized in any number of ways, such as by traits retained in the progeny, pedigree and/or molecular markers. Using the breeding methods described herein, one can develop individual plants, plant cells, and populations of plants that retain at least 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 99.5% genetic contribution from inbred line PH86T. In pedigree analysis the percentage genetic contribution may not be actually known, but on average 50% of the starting germplasm would be expected to be passed to the progeny line after one cross to another line, 25% after another cross to a different line, and so on. With backcrossing, the expected contribution of PH86T after 2, 3, 4 and 5 doses (or 1, 2, 3 and 4 backcrosses) would be 75%, 87.5%, 93.75% and 96.875% respectively. Actual genetic contribution may be much higher than the genetic contribution expected by pedigree, especially if molecular markers are used in selection. Molecular markers could also be used to confirm and/or determine the pedigree of the progeny line.

Specific methods and products produced using inbred line PH86T in plant breeding are discussed in the following sections. The methods outlined are described in detail by way of illustration and example for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications may be practiced within the scope of the invention.

One method for producing a line derived from inbred line PH86T is as follows. One of ordinary skill in the art would produce or obtain a seed from the cross between inbred line PH86T and another variety of maize, such as an elite inbred variety. The F1 seed derived from this cross would be grown to form a homogeneous population. The F1 seed would contain essentially all of the alleles from variety PH86T and essentially all of the alleles from the other maize variety. The F1 nuclear genome would be made-up of 50% variety PH86T and 50% of the other elite variety. The F1 seed would be grown and allowed to self, thereby forming F2 seed. On average the F2 seed would have derived 50% of its alleles from variety PH86T and 50% from the other maize variety, but many individual plants from the population would have a greater percentage of their alleles derived from PH86T (Wang J. and R. Bernardo, 2000, Crop Sci. 40:659-665 and Bernardo, R. and A. L. Kahler, 2001, Theor. Appl. Genet 102:986-992). The molecular markers of PH86T could be used to select and retain those lines with high similarity to PH86T. The F2 seed would be grown and selection of plants would be made based on visual observation, markers and/or measurement of traits. The traits used for selection may be any PH86T trait described in this specification, including the inbred per se maize PH86T traits described herein under the detailed description of inbred PH86T. Such traits may also be the good general or specific combining ability of PH86T, including its ability to produce hybrids with the approximate maturity and/or hybrid combination traits described herein in under the detailed description of inbred PH86T. The PH86T progeny plants that exhibit one or more of the desired PH86T traits, such as those listed herein, would be selected and each plant would be harvested separately. This F3 seed from each plant would be grown in individual rows and allowed to self. Then selected rows or plants from the rows would be harvested individually. The selections would again be based on visual observation, markers and/or measurements for desirable traits of the plants, such as one or more of the desirable PH86T traits listed herein. The process of growing and selection would be repeated any number of times until a PH86T progeny inbred plant is obtained. The PH86T progeny inbred plant would contain desirable traits derived from inbred plant PH86T, some of which may not have been expressed by the other maize variety to which inbred line PH86T was crossed and some of which may have been expressed by both maize varieties but now would be at a level equal to or greater than the level expressed in inbred variety PH86T. However, in each case the resulting progeny line would benefit from the efforts of the inventor(s), and would not have existed but for the inventor(s) work in creating PH86T. The PH86T progeny inbred plants would have, on average, 50% of their nuclear genes derived from inbred line PH86T, but many individual plants from the population would have a greater percentage of their alleles derived from PH86T. This breeding cycle, of crossing and selfing, and optional selection, may be repeated to produce another population of PH86T progeny maize plants with, on average, 25% of their nuclear genes derived from inbred line PH86T, but, again, many individual plants from the population would have a greater percentage of their alleles derived from PH86T. Another embodiment of the invention is a PH86T progeny plant that has received the desirable PH86T traits listed herein through the use of PH86T, which traits were not exhibited by other plants used in the breeding process.

The previous example can be modified in numerous ways, for instance selection may or may not occur at every selfing generation, selection may occur before or after the actual self-pollination process occurs, or individual selections may be made by harvesting individual ears, plants, rows or plots at any point during the breeding process described. In addition, double haploid breeding methods may be used at any step in the process. The population of plants produced at each and any cycle of breeding is also an embodiment of the invention, and on average each such population would predictably consist of plants containing approximately 50% of its genes from inbred line PH86T in the first breeding cycle, 25% of its genes from inbred line PH86T in the second breeding cycle, 12.5% of its genes from inbred line PH86T in the third breeding cycle and so on. However, in each case the use of PH86T provides a substantial benefit. The linkage groups of PH86T would be retained in the progeny lines, and since current estimates of the maize genome size is about 50,000-80,000 genes (Xiaowu, Gai et al., Nucleic Acids Research, 2000, Vol. 28, No. 1, 94-96), in addition to non-coding DNA that impacts gene expression, it provides a significant advantage to use PH86T as starting material to produce a line that retains desired genetics or traits of PH86T.

Another embodiment of this invention is the method of obtaining a substantially homozygous PH86T progeny plant by obtaining a seed from the cross of PH86T and another maize plant and applying double haploid methods to the F1 seed or F1 plant or to any successive filial generation. Such methods decrease the number of generations required to produce an inbred with similar genetics or characteristics to PH86T. See Bernardo, R. and Kahler, A. L., Theor. Appl. Genet. 102:986-992, 2001.

The utility of inbred maize line PH86T also extends to crosses with other species. Commonly, suitable species will be of the family Graminaceae, and especially of the genera Zea, Tripsacum, Coix, Schlerachne, Polytoca, Chionachne, and Trilobachne, of the tribe Maydeae. Potentially suitable for crosses with PH86T may be the various varieties of grain sorghum, Sorghum bicolor(L.) Moench.

As is readily apparent to one skilled in the art, the foregoing are only some of the various ways by which the inbred can be obtained by those looking to use the germplasm. Other means are available, and the above examples are illustrative only.

INDUSTRIAL APPLICABILITY

Maize is used as human food, livestock feed, and as raw material in industry. The food uses of maize, in addition to human consumption of maize kernels, include both products of dry- and wet-milling industries. The principal products of maize dry milling are grits, meal and flour. The maize wet-milling industry can provide maize starch, maize syrups, and dextrose for food use. Maize oil is recovered from maize germ, which is a by-product of both dry- and wet-milling industries.

Maize, including both grain and non-grain portions of the plant, is also used extensively as livestock feed, primarily for beef cattle, dairy cattle, hogs, and poultry.

Industrial uses of maize include production of ethanol, maize starch in the wet-milling industry and maize flour in the dry-milling industry. The industrial applications of maize starch and flour are based on functional properties, such as viscosity, film formation, adhesive properties, and ability to suspend particles. The maize starch and flour have application in the paper and textile industries. Other industrial uses include applications in adhesives, building materials, foundry binders, laundry starches, explosives, oil-well muds, and other mining applications.

Plant parts other than the grain of maize are also used in industry: for example, stalks and husks are made into paper and wallboard and cobs are used for fuel and to make charcoal.

The seed of inbred maize line PH86T, the plant produced from the inbred seed, the hybrid maize plant produced from the crossing of the inbred, hybrid seed, and various parts of the hybrid maize plant and transgenic versions of the foregoing, can be utilized for human food, livestock feed, and as a raw material in industry.

Deposits

Applicants have made a deposit of at least 2500 seeds of Inbred Maize Line PH86T with the American Type Culture Collection (ATCC), Manassas, Va. 201 10 USA, ATCC Deposit No. PTA-4869. The seeds deposited with the ATCC on Dec. 23, 2002 were taken from the deposit maintained by Pioneer Hi-Bred International, Inc., 800 Capital Square 400 Locust Street, Des Moines, Iowa 50309-2340 since prior to the filing date of this application. Access to this deposit will be available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. Upon allowance of any claims in the application, the Applicants will make the deposit available to the public pursuant to 37 C.F.R. §1.808. This deposit of the Inbred Maize Line PH86T will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicants have satisfy all of the requirements of 37 C.F.R. §§1.801-1.809, including providing an indication of the viability of the sample upon deposit. Applicants have no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicants do not waive any infringement of their rights granted under this patent or under the Plant Variety Protection Act (7 USC 2321 et seq.). U.S. Plant Variety Protection of Inbred Maize Line PH86T has been applied for under Application No. 200300223.

TABLE 1 VARIETY DESCRIPTION INFORMATION PH86T 1. TYPE: (Describe intermediate types in comments Sta. Sam. section) Avg. Dev Size 1 = Sweet, 2 = Dent, 3 = Flint, 4 = Flour, 5 = Pop and 3 6 = Ornamental. Comments: Flint-Dent 2. MATURITY: DAYS HEAT UNITS Days H. Units From emergence to 50% of plants in silk 62 1,573 From emergence to 50% of plants in pollen 61 1,553 From 10% to 90% pollen shed  2   49 From 50% Silk to harvest at 25% moisture 3. PLANT: Plant Height (to tassel tip) (cm) 263.2 17.97 15 Ear Height (to base of top ear node) (cm) 94.4 14.08 15 Length of Top Ear Internode (cm) 17.6 2.72 15 Average Number of Tillers per Plant 0.0 0.01 3 Average Number of Ears per Stalk 1.0 0.09 3 Anthocyanin of Brace Roots: 1 = Absent, 2 = Faint, 1 3 = Moderate, 4 = Dark 4. LEAF: Width of Ear Node Leaf (cm) 9.8 0.68 15 Length of Ear Node Leaf (cm) 83.8 4.92 15 Number of Leaves above Top Ear 6.3 1.10 15 Leaf Angle: (measure from 2nd leaf above 21.1 4.19 15 ear at anthesis to stalk above leaf) (Degrees) *Leaf color:  Dark Green  Munsell code:  7.5GY34 Leaf Sheath Pubescence: 1 = none to 9 = like peach fuzz 5 5. TASSEL: Number of Primary Lateral Branches 0.8 1.01 15 Branch Angle from Central Spike 7.6 8.72 15 Tassel Length (from top leaf collar to tassel tip) (cm): 63.5 3.81 15 Pollen Shed: 0 = male sterile, 9 = heavy shed 2 *Anther Color:  Yellow  Munsell code:  7.5Y8.58 *Glume Color:  Light Green Munsell code:  7.5GY46 *Bar Glumes (glume bands): 1 = absent, 2 = present 1 Peduncle Length (from top leaf to basal branches) (cm): 30.0 3.53 15 *Munsell Glossy Book of Color, a standard color reference Kollmorgen Inst. Corp. New Windsor NY. 6a. EAR (Unhusked ear) *Silk color:  Light Green Munsell c.  5GY78 (3 days after silk emergence) *Fresh husk color:  Light Green Munsell c.  5GY68 (25 days after 50% silking) *Dry husk color:  Buff  Munsell c.  2.5Y92 (65 days after 50% silking) Ear position at dry husk stage: 1 = upright, 2 = horizontal, 1 3 = pendant Husk tightness: (1 = very loose, 9 = very tight) 7 Husk extension (at harvest): 1 = short (ears exposed), 4 2 = medium (<8 cm), 3 = long (8-10 cm), 4 = v. long (>10 cm) 6b. EAR (Husked ear data) Ear length (cm): 13.6 1.24 15 Ear diameter at mid-point (mm) 47.6 2.03 15 Ear weight (gm): 132.3 31.57 15 Number of Kernel Rows: 15.7 0.70 15 Kernel Rows: 1 = indistinct, 2 = distinct 2 Row alignment: 1 = straight, 2 = slightly curved, 3 = spiral 2 Shank Length (cm): 9.0 1.85 15 Ear Taper: 1 = slight cylind., 2 = average, 3 = extreme conic. 2 7. KERNEL (Dried): Kernel Length (mm): 13.3 0.90 15 Kernel Width (mm): 10.0 0.38 15 Kernel Thickness (mm): 6.4 0.51 15 Round Kernels (shape grade) (%) 62.9 6.46 3 Aleurone Color pattern: 1 = homozygous, 2 = segregating 1 *Aleurone Color:  Yellow-Oran  Munsell c.:  10YR612 *Hard Endo. color:  Pink-Orange  Munsell c.:  10YR610 Endosperm Type: 3 1 = sweet (su1), 2 = extra sweet (sh2), 3 = normal starch, 4 = high amylose starch, 5 = waxy starch, 6 = high protein, 7 = high lysine, 8 = super sweet (se), 9 = high oil, 10 = other Weight per 100 kernels (unsized sample) (gm): 35.7 3.51 3 8. COB: *Cob Diameter at mid-point (mm): 28.7 1.11 15 *Cob Color:  White  Munsell c.:  5Y91 *Munsell Glossy Book of color, a standard color reference Kollmorgen Inst. Corp. New Windsor NY. 10. DISEASE RESISTANCE: (Rate from 1 = most-susceptable to 9 = most-resistant. Leave blank if not tested, leave race or strain options blank if polygenic.) A. LEAF BLIGHTS, WILTS, AND LOCAL INFECTION DISEASES Anthracnose Leaf Blight (Colletotrichum graminicola) 6 Common Rust (Puccinia sorghi) Common Smut (Ustilago maydis) Eyespot (Kabatiella zeae) Gross's Wilt (Clavibacter michiganense spp. nebraskense) 6 Gray Leaf Spot (Cercospora zeae-maydis) Helminthosporium Leaf Spot (Bipolaris zeicola) Race: 6 Northern Leaf Blight (Exserohilum turcicum) Race: 6 Southern Leaf Blight (Bipolaris maydis) Race: 4 Southern Rust (Puccinia polysora) 5 Stewart's Wilt (Erwinia stewartii) Other (Specify):      B. SYSTEMIC DISEASES Corn Lethal Necrosis (MCMV and MDMV) 8 Head Smut (Sphacelotheca reiliana) Maize Chlorotic Dwarf Virus (MDV) Maize Chlorotic Mottle Virus (MCMV) Maize Dwarf Mosaic Virus (MDMV) Sorghum Downy Mildew of Corn (Peronosclerospora sorghi) Other (Specify):      C. STALK ROTS 5 Anthracnose Stalk Rot (Colletotrichum graminicola) Diploidia Stalk Rot (Stenocarpella maydis) Fusarium Stalk Rot (Fusarium moniliforme) Gibberella Stalk Rot (Gibberella zeae) Other (Specify):      D. EAR AND KERNEL ROTS Aspergillus Ear and Kernel Rot (Aspergillus flavus) 5 Diplodia Ear Rot (Stenocarpella maydis) 7 Fusarium Ear and Kernel Rot (Fusarium moniliforme) 9 Gibberella Ear Rot (Gibberella zeae) Other (Specify):      *Munsell Glossy Book of Color, a Standard color reference Kollmorgrn Inst. Corp. New Windsor NY. 11. INSECT RESISTANCE: (Rate from 1 = most-suscept. to 9 = most-resist., leave blank if not tested.) Corn Worm (Helicoverpa zea) Leaf Feeding Silk Feeding Ear Damage Corn Leaf Aphid (Rophalosiphum maydis) Corn Sap Beetle (Capophilus dimidiatus) European Corn Borer (Ostrinia nubilalis) 4 1st. Generation (Typically whorl leaf feeding) 5 2nd. Generation (Typically leaf sheath-collar feeding) Stalk Tunneling cm tunneled/plant Fall armyworm (Spodoptera fruqiperda) Leaf Feeding Silk Feeding mg larval wt. Maize Weevil (Sitophilus zeamaize) Northern Rootworm (Diabrotica barberi) Southern Rootworm (Diabrotica undecimpunctata) Southwestern Corn Borer (Diatreaea grandiosella) Leaf Feeding Stalk Tunneling cm tunneled/plant Two-spotted Spider Mite (Tetranychus utricae) Western Rootworm (Diabrotica virgifrea virgifrera) Other (Specify):      12. AGRONOMIC TRAITS: 6 Staygreen (at 65 days after anthesis; rate from 1-worst to 9-excellent) 0 % Dropped Ears (at 65 days after anthesis) % Pre-anthesis Brittle Snapping 0 % Pre-anthesis Root Lodging 0 % Post-anthesis Root Lodging (at 65 days after anthesis) % Post-anthesis Stalk Lodging 5,081.0 Kg/ha Yield of inbred per se (at 12-13% grain moisture)

TABLE 2 SSR PROFILE OF PH86T Locus Bin # PH86T mwt bnlg1014  1.01 126 phi427913  1.01 131 phi056  1.01 257 bnlg1007  1.02 129 bnlg1429  1.02 203 bnlg1083  1.02 206 bnlg1627  1.02 209 bnlg1953  1.02 252 bnlg439  1.03 235 phi109275  1.03 147 phi339017  1.03 148 bnlg1484  1.03 148 bnlg1203  1.03 306 dupssr26  1.04 125 bnlg2086  1.04 222 bnlg2238  1.04 223 bnlg1016  1.04 236 bnlg1886  1.05 141 bnlg1832  1.05 236 bnlg1884  1.05 318 bnlg1041  1.06 197 bnlg1615  1.06 236 bnlg1556  1.07 181 phi423298  1.08 136 bnlg2228  1.08 248 phi323065  1.08 331 phi002  1.08 76 phi335539  1.08 91 bnlg1331  1.09 145 phi011  1.09 230 bnlg1597  1.09(1.10) 189 bnlg1720  1.09(1.10) 240 phi308707  1.10 134 phi265454  1.11 238 phi260485  1.11 288 phi227562  1.11 325 phi064  1.11 98 bnlg1130  1.XX 208 bnlg2031  1.XX 216 phi402893  2.00 215 bnlg1017  2.02 197 bnlg1327  2.02 303 bnlg2277  2.02 309 bnlg1537  2.03 124 phi109642  2.03 142 bnlg1064  2.03 191 bnlg1018  2.04 137 bnlg1909  2.05 308 bnlg1396  2.06 171 bnlg1036  2.06 185 bnlg1831  2.06 202 bnlg1138  2.06 221 phi251315  2.07 130 phi427434  2.08 124 phi127  2.08 126 bnlg1141  2.08 135 phi090  2.08 149 bnlg2237  2.08 220 bnlg1258  2.08 220 bnlg1940  2.08 239 bnlg1520  2.09 283 phi101049  2.10 238 phi453121  3.00 208 phi104127  3.01 164 phi404206  3.01 302 bnlg1144  3.02 118 phi193225  3.02 141 bnlg1647  3.02 160 phi374118  3.02 217 bnlg1523  3.03 268 bnlg1452  3.04 122 bnlg1113  3.04 133 phi029  3.04 154 bnlg1019  3.04 167 bnlg1035  3.05 104 phi053  3.05 169 phi073  3.05 188 bnlg1951  3.06 124 phi102228  3.06 139 bnlg2241  3.06 145 bnlg1160  3.06 223 bnlg1496  3.09 188 phi072  4.00(4.01) 151 phi295450  4.01 187 phi213984  4.01 304 bnlg1162  4.03 131 phi021  4.03 95 phi079  4.05 190 bnlg1265  4.05 196 phi438301  4.05 213 bnlg1755  4.05 243 bnlg1937  4.05(4.06) 230 bnlg1137  4.06 182 umc2038  4.07 125 bnlg1189  4.07 141 bnlg2244  4.08 204 phi093  4.08 291 bnlg1565  4.09 211 bnlg1890  4.11 204 bnlg1006  5.00 220 phi396160  5.02 303 phi109188  5.03 164 bnlg653  5.04 158 bnlg1208  5.04 121 phi331888  5.04 133 phi330507  5.04 135 bnlg1892  5.04 151 bnlg2323  5.04 200 phi333597  5.05 216 phi085  5.06 254 bnlg1711  5.07 179 bnlg1346  5.07 192 bnlg1118  5.07 86 bnlg1597  5.08 235 phi159819  6.00(6.08) 129 phi423796  6.01 131 bnlg1422  6.01 233 phi389203  6.03 307 phi445613  6.05 100 bnlg1174  6.05 222 umc1413  6.05 305 umc1463  6.06 300 phi299852  6.07 120 bnlg1759  6.07 128 phi364545  6.07 134 bnlg1740  6.07 234 phi070  6.07 86 phi338882  6.XX 169 bnlg2132  7.00 234 umc1159  7.01 232 phi034  7.02 123 bnlg1094  7.02 159 bnlg1292  7.03 141 bnlg1070  7.03 148 bnlg2271  7.03 237 phi328175  7.04 101 phi051  7.05 138 phi069  7.05 206 phi116  7.06 168 phi420701  8.00 294 bnlg1194  8.02 144 phi100175  8.03 140 bnlg2082  8.03 141 bnlg1863  8.03 245 phi115  8.03 291 phi121  8.03 98 bnlg2046  8.04 309 bnlg1152  8.06 151 bnlg1031  8.06 293 bnlg1828  8.07 186 bnlg1065  8.07 231 bnlg1056  8.08 113 phi015  8.08 82 phi233376  8.09 155 bnlg1810  9.01 200 bnlg2122  9.01 240 phi033  9.01 252 umc1037  9.02 197 bnlg1012  9.04 162 phi032  9.04 241 phi236654  9.05 120 phi108411  9.05 129 phi448880  9.06(9.07) 187 bnlg619  9.07 266 bnlg1375  9.07 168 bnlg1129  9.08 302 phi041 10.00 205 phi059 10.02 156 phi96342 10.02 249 bnlg1037 10.03 121 bnlg1079 10.03 165 bnlg1655 10.03 207 phi050 10.03 84 phi301654 10.04 138 phi062 10.04 164 phi323152 10.05 147 bnlg1074 10.05 186 bnlg1028 10.06 145 bnlg1185 10.07 191 bnlg1839 10.07 208 bnlg1450 10.07 242

TABLE 3A PAIRED INBRED COMPARISON REPORT Variety #1: PH86T Variety #2: PH07D YIELD YIELD MST TSTWT EGRWTH ESTCNT TILLER GDUSHD GDUSLK POLWT BU/A 56# BU/A 56# PCT LB/BU SCORE COUNT PCT GDU GDU VALUE Stat ABS % MN ABS ABS ABS ABS ABS ABS ABS ABS Mean1 82.4 106.7 23.8 56.2 6.5 24.4 1.7 152.0 153.7 72.9 Mean2 75.2 98.4 20.5 56.3 6.2 23.8 1.1 149.2 151.9 95.8 Locs 49 49 51 9 27 25 32 44 44 4 Reps 53 53 55 9 27 25 32 44 44 8 Diff 7.2 8.2 −3.3 0.0 0.3 0.6 −0.7 2.8 1.8 −22.9 Prob 0.059 0.106 0.000 0.935 0.142 0.600 0.255 0.000 0.010 0.207 POLWT TASBLS TASSZ PLTHT EARHT STAGRN STKLDG BRTSTK SCTGRN EARSZ VALUE SCORE SCORE CM CM SCORE % NOT % NOT SCORE SCORE Stat % MN ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 68.9 8.4 2.3 250.3 99.7 6.3 100.0 100.0 6.5 4.3 Mean2 86.1 9.0 3.7 238.0 92.7 5.8 99.3 98.3 7.1 4.0 Locs 4 7 36 36 4 6 6 1 12 3 Reps 8 7 36 36 4 6 6 1 12 3 Diff −17.2 −0.6 −1.4 12.3 7.0 0.5 0.7 1.7 −0.6 0.3 Prob 0.271 0.356 0.000 0.000 0.205 0.363 0.363 . 0.294 0.742 TEXEAR EARMLD BARPLT DRPEAR GLFSPT NLFBLT SLFBLT STWWLT ANTROT FUSERS SCORE SCORE % NOT % NOT SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 7.0 7.6 96.1 100.0 6.1 5.8 6.8 5.0 4.0 7.3 Mean2 6.8 7.3 93.8 100.0 6.2 5.8 4.5 4.0 4.5 6.6 Locs 5 9 35 1 5 2 2 1 1 13 Reps 5 9 35 1 7 4 3 1 2 15 Diff 0.2 0.2 2.4 0.0 −0.1 0.0 2.3 1.0 −0.5 0.7 Prob 0.374 0.681 0.130 . 0.854 1.000 0.070 . . 0.126 DIPERS COMRST SOURST CLDTST CLDTST KSZDCD ERTLDG LRTLDG ERTLPN LRTLPN SCORE SCORE SCORE PCT PCT PCT % NOT % NOT % NOT % NOT Stat ABS ABS ABS ABS % MN ABS ABS ABS ABS ABS Mean1 4.5 6.4 5.0 91.6 105.7 4.5 29.2 94.5 100.0 100.0 Mean2 3.7 6.8 5.0 92.2 106.6 6.9 95.5 99.1 100.0 100.0 Locs 3 5 1 23 23 23 1 2 1 1 Reps 4 5 1 23 23 23 1 2 1 1 Diff 0.8 −0.4 0.0 −0.7 −0.9 −2.4 66.3 −4.6 0.0 0.0 Prob 0.199 0.178 . 0.433 0.385 0.071 . 0.339 . .

TABLE 3B PAIRED INBRED COMPARISON REPORT Variety #1: PH86T Variety #2: PH2JR YIELD YIELD MST TSTWT EGRWTH ESTCNT TILLER GDUSHD GDUSLK BU/A 56# BU/A 56# PCT LB/BU SCORE COUNT PCT GDU GDU Stat ABS % MN ABS ABS ABS ABS ABS ABS ABS Mean1 65.0 93.9 22.7 57.1 6.4 21.3 1.1 150.1 151.6 Mean2 70.3 103.7 21.6 56.1 5.9 22.1 2.5 149.4 151.1 Locs 22 22 23 4 27 20 22 48 48 Reps 22 22 23 4 27 20 22 48 48 Diff −5.2 −9.9 −1.0 1.1 0.5 −0.8 1.5 0.7 0.5 Prob 0.386 0.354 0.237 0.458 0.045 0.314 0.237 0.257 0.480 TASBLS TASSZ PLTHT EARHT STAGRN STKLDG BRTSTK SCTGRN EARSZ SCORE SCORE CM CM SCORE % NOT % NOT SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 8.4 2.3 252.2 105.8 6.1 100.0 100.0 6.3 4.3 Mean2 9.0 5.2 224.1 77.9 6.6 95.8 96.6 6.9 5.3 Locs 7 42 41 3 10 6 1 14 3 Reps 7 42 41 3 10 6 1 14 3 Diff −0.6 −2.9 28.1 27.9 −0.5 4.2 3.4 −0.6 −1.0 Prob 0.356 0.000 0.000 0.053 0.427 0.363 . 0.133 0.423 TEXEAR EARMLD BARPLT DRPEAR GLFSPT NLFBLT SLFBLT ANTROT MDMCPX SCORE SCORE % NOT % NOT SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 7.2 7.5 94.6 100.0 5.8 6.3 6.3 5.0 2.8 Mean2 6.3 5.4 96.9 100.0 4.8 5.9 6.9 5.4 2.6 Locs 6 10 31 1 12 5 6 8 4 Reps 6 10 32 1 20 10 10 15 8 Diff 0.8 2.1 −2.4 0.0 1.0 0.4 −0.7 −0.4 0.1 Prob 0.141 0.001 0.067 . 0.004 0.338 0.043 0.176 0.638 FUSERS GIBERS DIPERS COMRST SOURST ECBILF ECB2SC CLDTST CLDTST SCORE SCORE SCORE SCORE SCORE SCORE SCORE PCT PCT Stat ABS ABS ABS ABS ABS ABS ABS ABS % MN Mean1 7.2 9.0 4.6 6.3 3.0 4.0 5.0 88.3 103.5 Mean2 4.3 9.0 3.6 4.6 4.0 5.0 4.5 87.9 103.1 Locs 19 1 4 6 1 1 1 10 10 Reps 24 1 7 7 1 2 2 10 10 Diff 2.8 0.0 1.0 1.7 −1.0 −1.0 0.5 0.4 0.4 Prob 0.000 . 0.066 0.020 . . . 0.863 0.885 HD KSZDCD SMT ERTLDG ERTLPN LRTLPN PCT % NOT % NOT % NOT % NOT Stat ABS ABS ABS ABS ABS Mean1 4.9 84.8 29.2 100.0 100.0 Mean2 3.8 100.0 25.0 100.0 100.0 Locs 10 3 1 1 1 Reps 10 6 1 1 1 Diff 1.1 −15.2 4.2 0.0 0.0 Prob 0.519 0.221 . . .

TABLE 3C PAIRED INBRED COMPARISON REPORT Variety #1: PH86T Variety #2: PHMK0 YIELD YIELD MST EGRWTH ESTCNT TILLER GDUSHD GDUSLK BU/A 56# BU/A 56# PCT SCORE COUNT PCT GDU GDU Stat ABS % MN ABS ABS ABS ABS ABS ABS Mean1 48.8 83.7 22.4 6.4 31.0 0.0 155.3 158.0 Mean2 63.2 106.8 25.9 4.2 29.0 0.0 158.5 165.5 Locs 10 10 10 5 3 4 10 10 Reps 10 10 10 5 3 4 10 10 Diff −14.4 −23.1 3.5 2.2 2.0 0.0 −3.2 −7.5 Prob 0.117 0.147 0.013 0.020 0.368 1.000 0.026 0.000 TASSZ PLTHT STAGRN STKLDG SCTGRN TEXEAR EARMLD BARPLT SCORE CM SCORE % NOT SCORE SCORE SCORE % NOT Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 2.5 241.3 5.3 100.0 5.5 7.5 7.0 88.7 Mean2 4.2 230.3 7.7 93.8 3.0 5.0 6.0 87.7 Locs 10 6 3 2 2 2 1 8 Reps 10 6 3 2 2 2 1 8 Diff −1.7 11.0 −2.3 6.3 2.5 2.5 1.0 1.0 Prob 0.006 0.236 0.020 0.500 0.344 0.126 . 0.734 DRPEAR GLFSPT NLFBLT SLFBLT ANTROT MDMCPX FUSERS DIPERS % NOT SCORE SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 100.0 5.4 7.0 5.8 5.0 2.8 6.5 6.0 Mean2 100.0 6.5 7.0 7.3 6.4 2.5 6.3 6.0 Locs 1 5 2 2 4 2 2 2 Reps 1 9 4 4 7 4 3 3 Diff 0.0 −1.1 0.0 −1.5 −1.4 0.3 0.3 0.0 Prob . 0.030 1.000 0.205 0.223 0.500 0.795 1.000 HD COMRST ECB1LF ECB2SC CLDTST CLDTST KSZDCD SMT ERTLDG SCORE SCORE SCORE PCT PCT PCT % NOT % NOT Stat ABS ABS ABS ABS % MN ABS ABS ABS Mean1 5.8 4.0 5.0 85.0 103.3 4.6 85.0 29.2 Mean2 6.7 7.5 7.0 85.6 104.0 7.4 97.9 100.0 Locs 3 1 1 5 5 5 2 1 Reps 4 2 2 5 5 5 4 1 Diff −0.8 −3.5 −2.0 −0.6 −0.7 −2.8 −12.9 −70.8 Prob 0.199 . . 0.778 0.791 0.148 0.500 .

TABLE 3D PAIRED INBRED COMPARISON REPORT Variety #1: PH86T Variety #2: PHKV1 YIELD YIELD MST TSTWT EGRWTH ESTCNT TILLER GDUSHD GDUSLK BU/A 56# BU/A 56# PCT LB/BU SCORE COUNT PCT GDU GDU Stat ABS % MN ABS ABS ABS ABS ABS ABS ABS Mean1 83.2 107.4 23.9 55.9 6.3 24.7 0.9 152.4 154.0 Mean2 58.6 73.1 22.0 55.4 5.7 26.0 2.2 155.4 158.2 Locs 47 47 49 9 26 21 27 51 51 Reps 51 51 53 9 26 21 27 51 51 Diff 24.5 34.3 −1.9 0.5 0.6 −1.3 1.3 −3.0 −4.2 Prob 0.000 0.000 0.004 0.632 0.003 0.252 0.300 0.000 0.000 POLWT POLWT TASBLS TASSZ PLTHT EARHT STAGRN STKLDG BRTSTK VALUE VALUE SCORE SCORE CM CM SCORE % NOT % NOT Stat ABS % MN ABS ABS ABS ABS ABS ABS ABS Mean1 72.9 68.9 8.4 2.1 250.4 96.5 6.3 100.0 100.0 Mean2 87.3 79.1 9.0 2.5 236.3 94.5 3.0 100.0 98.3 Locs 4 4 7 46 45 5 9 6 1 Reps 8 8 7 46 45 5 9 6 1 Diff −14.4 −10.2 −0.6 −0.4 14.2 2.0 3.3 0.0 1.7 Prob 0.560 0.617 0.356 0.015 0.000 0.757 0.001 1.000 . SCTGRN EARSZ TEXEAR EARMLD BARPLT DRPEAR GLFSPT NLFBLT SLFBLT SCORE SCORE SCORE SCORE % NOT % NOT SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 6.3 4.5 7.3 8.1 94.7 100.0 5.5 6.3 6.4 Mean2 6.8 3.3 7.7 7.6 84.3 100.0 2.5 3.5 5.5 Locs 16 4 7 8 36 1 12 5 7 Reps 16 4 7 8 37 1 20 10 11 Diff −0.4 1.3 −0.4 0.5 10.4 0.0 3.1 2.8 0.9 Prob 0.417 0.015 0.200 0.451 0.004 . 0.000 0.007 0.193 STWWLT ANTROT MDMCPX FUSERS GIBERS DIPERS COMRST SOURST ECB1LF SCORE SCORE SCORE SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 5.0 5.0 2.8 7.4 9.0 4.6 6.3 4.0 4.0 Mean2 6.0 3.1 3.3 7.6 9.0 4.1 3.7 3.5 5.5 Locs 1 8 4 17 1 4 6 2 1 Reps 1 15 8 22 1 7 7 2 2 Diff −1.0 1.9 −0.5 −0.2 0.0 0.5 2.6 0.5 −1.5 Prob . 0.075 0.308 0.299 . 0.594 0.008 0.500 . HD ECB2SC CLDTST CLDTST KSZDCD SMT ERTLDG LRTLDG ERTLPN LRTLPN SCORE PCT PCT PCT % NOT % NOT % NOT % NOT % NOT Stat ABS ABS % MN ABS ABS ABS ABS ABS ABS Mean1 5.0 90.9 105.4 4.5 84.8 29.2 94.5 100.0 100.0 Mean2 2.0 89.8 104.2 2.6 97.8 58.3 91.4 95.0 100.0 Locs 1 24 24 23 3 1 2 2 1 Reps 2 24 24 23 6 1 2 2 1 Diff 3.0 1.1 1.2 1.8 −13.0 −29.2 3.1 5.0 0.0 Prob . 0.563 0.606 0.044 0.281 . 0.659 0.500 .

TABLE 3E PAIRED INBRED COMPARISON REPORT Variety #1: PH86T Variety #2: PH6CF YIELD YIELD MST TSTWT EGRWTH ESTCNT TILLER GDUSHD GDUSLK BU/A 56# BU/A 56# PCT LB/BU SCORE COUNT PCT GDU GDU Stat ABS % MN ABS ABS ABS ABS ABS ABS ABS Mean1 78.3 102.9 23.5 55.7 6.4 24.0 1.5 151.2 152.8 Mean2 59.2 77.7 24.1 55.3 6.1 24.3 0.5 146.9 150.4 Locs 47 47 49 7 28 23 31 51 51 Reps 51 51 53 7 28 23 31 51 51 Diff 19.1 25.2 0.6 0.4 0.3 −0.4 −1.0 4.3 2.4 Prob 0.000 0.000 0.126 0.464 0.131 0.661 0.051 0.000 0.001 POLWT POLWT TASBLS TASSZ PLTHT EARHT STAGRN STKLDG BRTSTK VALUE VALUE SCORE SCORE CM CM SCORE % NOT % NOT Stat ABS % MN ABS ABS ABS ABS ABS ABS ABS Mean1 72.9 68.9 8.4 2.3 252.0 104.1 6.1 100.0 100.0 Mean2 126.7 117.4 9.0 4.6 229.0 84.5 5.9 100.0 100.0 Locs 4 4 7 42 42 4 10 6 1 Reps 7 7 7 42 42 4 10 6 1 Diff −53.8 −48.6 −0.6 −2.4 23.0 19.7 0.2 0.0 0.0 Prob 0.044 0.038 0.356 0.000 0.000 0.045 0.716 1.000 . SCTGRN EARSZ TEXEAR EARMLD BARPLT DRPEAR GLFSPT NLFBLT SLFBLT SCORE SCORE SCORE SCORE % NOT % NOT SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 6.3 4.3 7.2 7.5 94.9 100.0 5.8 6.0 6.3 Mean2 5.7 4.7 6.8 6.9 85.3 100.0 4.5 6.2 7.9 Locs 14 3 6 10 34 1 8 3 4 Reps 14 3 6 10 34 1 12 6 6 Diff 0.6 −0.3 0.3 0.6 9.6 0.0 1.3 −0.2 −1.6 Prob 0.205 0.667 0.175 0.260 0.001 . 0.002 0.423 0.061 STWWLT ANTROT MDMCPX FUSERS GIBERS DIPERS COMRST SOURST ECB1LF SCORE SCORE SCORE SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS ABS Mean1 5.0 5.5 2.5 7.3 9.0 4.6 6.3 3.0 4.0 Mean2 4.0 5.8 3.5 6.5 9.0 2.4 5.3 7.0 5.0 Locs 1 3 1 14 1 4 6 1 1 Reps 1 6 2 16 1 6 7 1 2 Diff 1.0 −0.3 −1.0 0.9 0.0 2.3 1.0 −4.0 −1.0 Prob . 0.423 . 0.045 . 0.006 0.012 . . HD ECB2SC CLDTST CLDTST KSZDCD SMT ERTLDG LRTLDG ERTLPN LRTLPN SCORE PCT PCT PCT % NOT % NOT % NOT % NOT % NOT Stat ABS ABS % MN ABS ABS ABS ABS ABS ABS Mean1 5.0 90.8 105.5 4.0 85.0 29.2 94.5 100.0 100.0 Mean2 4.0 87.2 101.2 2.1 100.0 66.7 100.0 100.0 100.0 Locs 1 23 23 22 2 1 2 1 1 Reps 2 23 23 22 4 1 2 1 1 Diff 1.0 3.6 4.3 1.9 −15.0 −37.5 −5.5 0.0 0.0 Prob . 0.005 0.004 0.003 0.500 . 0.370 . .

TABLE 4A Average Inbred By Tester Performance Comparing PH86T To PH2JR Crossed To The Same Inbred Testers And Grown In The Same Experiments. TSTWT YIELD MST EGRWTH ESTCNT GDUSHD GDUSLK PLTHT EARHT STAGRN LB/BU — BU/A 56# PCT SCORE COUNT GDU GDU CM CM SCORE ABS Reps 194 197 31 30 5 4 42 42 71 156 Locs 194 197 31 30 5 4 42 42 71 156 PH86T 186.2 20.4 5.8 50.6 140.6 137.7 328.8 143.9 5.5 56.2 PH2JR 182.7 20.6 5.1 49.9 140.2 137.5 317.9 139.0 4.7 57.1 Diff 3.5 0.2 0.7 0.7 0.4 0.2 10.9 4.9 0.7 0.9 Pr > T 0.099 0.069 0.004 0.265 0.854 0.943 0.000 0.030 0.000 0.000 GLFSPT NLFBLT SLFBLT ANTROT COMRST ECB1LF EBTSTK SCORE SCORE SCORE SCORE SCORE SCORE % NOT — ABS ABS ABS ABS ABS ABS ABS Reps 11 2 7 13 4 3 30 Locs 11 2 7 13 4 3 30 PH86T 6.0 5.5 5.2 3.9 4.8 3.2 67.9 PH2JR 5.5 7.0 6.7 4.2 3.8 2.2 82.3 Diff 0.5 1.5 1.5 0.3 1.0 1.0 14.3 Pr > T 0.137 0.374 0.009 0.518 0.321 0.009 *Pr > T values are valid only for comparisons with Locs >= 10

TABLE 4B Average Inbred By Tester Performance Comparing PH86T To PH6CF Crossed To The Same Inbred Testers And Grown In The Same Experiments TSTWT GLFSPT GIBROT YIELD MST EGRWTH ESTCNT PLTHT EARHT STAGRN LB/BU SCORE SCORE — BU/A 56# PCT SCORE COUNT CM CM SCORE ABS ABS ABS Reps 20 21 3 3 2 2 6 15 1 1 Locs 20 21 3 3 2 2 6 15 1 1 PH86T 195.7 22.4 5.7 50.7 334.0 148.6 5.7 55.7 5.0 3.0 PH6CF 174.2 22.5 6.0 51.7 302.3 129.5 4.8 57.6 5.0 5.0 Diff 21.5 0.2 0.3 1.0 31.7 19.1 0.8 1.8 0.0 2.0 Pr > T 0.005 0.699 0.423 0.622 0.126 0.278 0.448 0.003 *Pr > T values are valid only for comparisons with Locs >= 10

TABLE 5 General Combining Ability Report for Inbred PH86T YIELD bu/a 56# ABS Mean 187.1 YIELD bu/a 56# ABS Locs 169 YIELD bu/a 56# ABS Reps 1208 YIELD bu/a 56# ABS Years 4 MST pct ABS Mean 20 MST pct ABS Locs 171 MST pct ABS Reps 1224 MST pct ABS Years 4 EGRWTH score ABS Mean 6 EGRWTH score ABS Locs 27 EGRWTH score ABS Reps 179 EGRWTH score ABS Years 4 ESTCNT count ABS Mean 52.3 ESTCNT count ABS Locs 21 ESTCNT count ABS Reps 245 ESTCNT count ABS Years 3 GDUSHD GDU ABS Mean 143.3 GDUSHD GDU ABS Locs 30 GDUSHD GDU ABS Reps 50 GDUSHD GDU ABS Years 3 GDUSLK GDU ABS Mean 143.3 GDUSLK GDU ABS Locs 23 GDUSLK GDU ABS Reps 38 GDUSLK GDU ABS Years 3 PLTHT cm ABS Mean 331.6 PLTHT cm ABS Locs 38 PLTHT cm ABS Reps 303 PLTHT cm ABS Years 4 EARHT cm ABS Mean 147.4 EARHT cm ABS Locs 38 EARHT cm ABS Reps 302 EARHT cm ABS Years 4 STAGRN score ABS Mean 5.9 STAGRN score ABS Locs 55 STAGRN score ABS Reps 449 STAGRN score ABS Years 4 TSTWT lb/bu ABS Mean 56 TSTWT lb/bu ABS Locs 151 TSTWT lb/bu ABS Reps 1041 TSTWT lb/bu ABS Years 4 GLFSPT score ABS Mean 6.1 GLFSPT score ABS Locs 14 GLFSPT score ABS Reps 91 GLFSPT score ABS Years 4 NLFBLT score ABS Mean 5.3 NLFBLT score ABS Locs 6 NLFBLT score ABS Reps 20 NLFBLT score ABS Years 3 SLFBLT score ABS Mean 5.2 SLFBLT score ABS Locs 8 SLFBLT score ABS Reps 89 SLFBLT score ABS Years 4 ANTROT score ABS Mean 4.5 ANTROT score ABS Locs 13 ANTROT score ABS Reps 105 ANTROT score ABS Years 3 FUSERS score ABS Mean 6.6 FUSERS score ABS Locs 7 FUSERS score ABS Reps 17 FUSERS score ABS Years 3 DIPERS score ABS Mean 4 DIPERS score ABS Locs 3 DIPERS score ABS Reps 10 DIPERS score ABS Years 2 COMRST score ABS Mean 4.5 COMRST score ABS Locs 1 COMRST score ABS Reps 21 COMRST score ABS Years 1 SOURST score ABS Mean 3.8 SOURST score ABS Locs 1 SOURST score ABS Reps 25 SOURST score ABS Years 1 ECB1LF score ABS Mean 4.3 ECB1LF score ABS Locs 3 ECB1LF score ABS Reps 10 ECB1LF score ABS Years 2 ECB2SC score ABS Mean 3.6 ECB2SC score ABS Locs 3 ECB2SC score ABS Reps 10 ECB2SC score ABS Years 2 GIBROT score ABS Mean 5.7 GIBROT score ABS Locs 2 GIBROT score ABS Reps 6 GIBROT score ABS Years 1 BRTSTK % NOT ABS Mean 100 BRTSTK % NOT ABS Locs 1 BRTSTK % NOT ABS Reps 2 BRTSTK % NOT ABS Years 1 STLLPN % NOT ABS Mean 72.3 STLLPN % NOT ABS Locs 29 STLLPN % NOT ABS Reps 261 STLLPN % NOT ABS Years 4 DIPROT score ABS Mean 4.3 DIPROT score ABS Locs 1 DIPROT score ABS Reps 4 DIPROT score ABS Years 1 EBTSTK % NOT ABS Mean 95.4 EBTSTK % NOT ABS Locs 3 EBTSTK % NOT ABS Reps 54 EBTSTK % NOT ABS Years 1 ABTSTK % NOT ABS Mean 70.6 ABTSTK % NOT ABS Locs 12 ABTSTK % NOT ABS Reps 67 ABTSTK % NOT ABS Years 3 ERTLPN % NOT ABS Mean 74.9 ERTLPN % NOT ABS Locs 14 ERTLPN % NOT ABS Reps 121 ERTLPN % NOT ABS Years 2 LRTLPN % NOT ABS Mean 68.8 LRTLPN % NOT ABS Locs 9 LRTLPN % NOT ABS Reps 17 LRTLPN % NOT ABS Years 1

TABLE 5A Combining Ability Report for Inbred PH86T F1 data for Inbred Line PH86T when crossed to various tester lines YIELD YIELD YIELD bu/a 56# bu/a 56# bu/a 56# MST pct MST pct MST pct ABS ABS ABS ABS ABS ABS Tester Mean Locs Reps Mean Locs Reps 61678B 202.3 16 16 19.1 16 16 72268A 179.9 16 16 18 16 16 70783D 171.5 16 16 19 16 16 56411E 194.3 29 33 21.7 29 33 62743E 176.8 16 16 20.5 16 16 62906B 183.8 17 22 19.3 17 22 70784B 172.5 14 18 21.1 15 19 198648K 210.4 14 14 19.1 15 15 51428E 198.2 15 15 18.7 15 15 58724D 196.3 30 30 19.9 31 31 58725E 188.4 15 15 19.5 16 16 58729C 168.2 15 15 19.3 15 15 58731B 182.6 16 16 19.4 16 16 61652C 205.2 16 16 19.5 16 16 61677A 207.8 15 15 20.4 15 15 61700D 202.3 17 22 19.4 17 22 198648F 187.7 16 16 19.1 16 16 62703D 187.8 14 14 19.7 15 15 70784E 206.9 14 14 20 14 14 197496H 202.7 15 15 19.3 15 15 197496J 198.8 14 14 18.5 15 15 198649B 194.2 13 13 20 14 14 56531H 177.7 31 31 20.3 32 32 50075F 172.5 18 18 21.6 18 18 53301F 220 14 14 19 14 14 51410K 195.1 15 15 19.4 16 16 53292D 172.2 17 17 20 17 17 56410C 192.7 14 14 18.8 14 14 EGRWTH EGRWTH EGRWTH ESTCNT ESTCNT ESTCNT score ABS score ABS score ABS count ABS count ABS count ABS Tester Mean Locs Reps Mean Locs Reps 61678B 6 1 1 72268A 6.2 6 6 45.3 4 4 70783D 6 5 5 47.3 4 4 56411E 6 3 3 48 4 4 62743E 6.4 5 5 46 4 4 62906B 4.5 1 2 70784B 6 2 2 47.8 4 4 198648K 7 1 1 51428E 5 1 1 58724D 6.7 6 6 48 4 4 58725E 6.6 5 5 47.8 4 4 58729C 6.3 4 4 44 3 3 58731B 6.4 5 5 46.5 4 4 61652C 7 1 1 61677A 6 1 1 61700D 6 1 2 198648F 5 1 1 62703D 7.2 5 5 47.8 4 4 70784E 6 1 1 197496H 4 1 1 197496J 5 1 1 198649B 5 1 1 56531H 6 9 9 50.1 8 8 50075F 6.8 4 4 53.5 4 4 53301F 5 1 1 51410K 5.8 5 5 47.5 4 4 53292D 6 4 4 51.3 4 4 56410C 6 5 5 46.8 4 4 PLTHT cm PLTHT cm PLTHT cm EARHT EARHT EARHT ABS ABS ABS cm ABS cm ABS cm ABS Tester Mean Locs Reps Mean Locs Reps 61678B 352.4 4 4 158.1 4 4 72268A 307.3 4 4 138.4 4 4 70783D 316.2 4 4 141 4 4 56411E 335.8 8 9 152.7 8 9 62743E 307.3 4 4 130.8 4 4 62906B 327.7 4 4 152.4 3 3 70784B 314.5 5 6 141.8 5 6 198648K 360 4 4 160.7 4 4 51428E 334.6 4 4 148 4 4 58724D 329.6 8 8 148 8 8 58725E 313.1 4 4 131.4 4 4 58729C 302.3 2 2 151.1 2 2 58731B 311.8 4 4 135.3 4 4 61652C 354.3 4 4 158.1 4 4 61677A 339.7 4 4 149.9 4 4 61700D 313.1 4 4 154.9 4 4 198648F 353.1 4 4 166.4 4 4 62703D 318.8 4 4 143.5 4 4 70784E 328.9 4 4 143.5 4 4 197496H 342.9 4 4 154.3 4 4 197496J 355.6 4 4 162.6 4 4 198649B 332.7 4 4 147.3 4 4 56531H 313.1 8 8 131.1 8 8 50075F 325.1 4 4 139.1 4 4 53301F 345.4 4 4 162.6 4 4 51410K 315.6 4 4 144.8 4 4 53292D 308 4 4 132.1 4 4 56410C 304.8 4 4 131.4 4 4 STAGRN STAGRN STAGRN TSTWT TSTWT TSTWT score ABS score ABS score ABS lb/bu ABS lb/bu ABS lb/bu ABS Tester Mean Locs Reps Mean Locs Reps 61678B 6.2 6 6 57.4 12 12 72268A 4.6 5 5 57.4 15 15 70783D 5 6 6 56.9 15 15 56411E 7 10 10 55.4 24 28 62743E 6.5 6 6 57.5 15 15 62906B 6.6 7 9 55.5 12 15 70784B 7.4 5 5 56.1 14 18 198648K 6.2 6 6 54.8 12 12 51428E 5.5 6 6 56.9 11 11 58724D 5.9 12 12 57.7 26 26 58725E 5.8 6 6 56.4 15 15 58729C 4.8 5 5 56.7 14 14 58731B 4.8 6 6 57.1 15 15 61652C 4.8 6 6 56.8 12 12 61677A 7.2 6 6 56.4 11 11 61700D 6.4 7 9 56.3 11 14 198648F 5.7 6 6 56.3 12 12 62703D 6.4 5 5 57.4 14 14 70784E 7.3 6 6 56 10 10 197496H 5.8 6 6 57.3 11 11 197496J 6.2 6 6 57.4 11 11 198649B 5.5 6 6 57 11 11 56531H 5.9 10 10 57.3 26 26 50075F 5.8 6 6 56.8 13 13 53301F 5.2 6 6 56.7 10 10 51410K 6 5 5 56.3 15 15 53292D 5.2 5 5 56.5 12 12 56410C 6.2 5 5 58.3 13 13 GLFSPT GLFSPT GLFSPT SLFBLT SLFBLT SLFBLT score ABS score ABS score ABS score ABS score ABS score ABS Tester Mean Locs Reps Mean Locs Reps 61678B 7 2 2 5.5 2 2 72268A 70783D 56411E 5 2 2 3 2 2 62743E 62906B 5.7 4 6 70784B 198648K 5.5 2 2 7 2 2 51428E 6 2 2 3.5 2 2 58724D 6.5 2 2 5.5 2 2 58725E 58729C 58731B 61652C 7 2 2 4 2 2 61677A 8 2 2 5 2 2 61700D 4.5 4 6 198648F 6.5 2 2 5 2 2 62703D 70784E 7.5 2 2 4.5 2 2 197496H 6 2 2 5 2 2 197496J 6.5 2 2 6.5 2 2 198649B 6.5 2 2 6.5 2 2 56531H 5 1 1 5 1 1 50075F 6 1 1 6 1 1 53301F 6 2 2 5 2 2 51410K 53292D 5 1 1 5 1 1 56410C ANTROT ANTROT ANTROT COMRST COMRST COMRST score ABS score ABS score ABS score ABS score ABS score ABS Tester Mean Locs Reps Mean Locs Reps 61678B 4.5 2 2 72268A 3 1 1 4 1 1 70783D 5 1 1 5 1 1 56411E 6 3 3 5.5 1 2 62743E 5 1 1 5 1 1 62906B 5 2 2 70784B 7 1 1 4 1 2 198648K 4.5 2 2 51428E 3.5 2 2 58724D 4.3 3 3 5 1 1 58725E 5 1 1 3 1 1 58729C 4 1 1 58731B 3 1 1 4 1 1 61652C 3.5 2 2 61677A 4.5 2 2 61700D 4.5 2 2 198648F 4 2 2 62703D 6 1 1 4 1 1 70784E 4 2 2 197496H 3.5 2 2 197496J 4.5 2 2 198649B 4.5 2 2 56531H 7 1 1 3 1 1 50075F 53301F 4 2 2 51410K 3 1 1 5 1 1 53292D 56410C 7 1 1 5 1 1 STLLPN STLLPN STLLPN EBTSTK EBTSTK EBTSTK % NOT % NOT % NOT % NOT % NOT % NOT ABS ABS ABS ABS ABS ABS Tester Mean Locs Reps Mean Locs Reps 61678B 78.6 3 3 72268A 56.9 2 2 98.1 3 3 70783D 98.1 2 2 92 3 3 56411E 85.3 4 4 93.2 3 3 62743E 55.3 2 2 98.4 3 3 62906B 94.8 2 2 70784B 70.6 1 1 95.1 3 3 198648K 72.7 3 3 51428E 66.8 3 3 58724D 71.3 5 5 98.7 3 3 58725E 65.5 2 2 90 3 3 58729C 94.1 1 1 100 2 2 58731B 78 2 2 95.2 3 3 61652C 66.6 3 3 61677A 81.9 3 3 61700D 69.4 2 2 198648F 69.6 3 3 62703D 87.8 2 2 92.2 3 3 70784E 79.2 3 3 197496H 72.8 3 3 197496J 70.9 3 3 198649B 82.3 3 3 56531H 74 5 5 94.5 3 3 50075F 75.6 3 3 53301F 51.7 3 3 51410K 81.2 2 2 96.9 3 3 53292D 79.3 3 3 56410C 98.1 2 2 92.7 3 3 ERTLPN ERTLPN ERTLPN % NOT % NOT % NOT ABS ABS ABS Tester Mean Locs Reps 61678B 66.7 3 3 72268A 70783D 56411E 85 2 2 62743E 62906B 48 3 5 70784B 198648K 80 3 3 51428E 80 3 3 58724D 85 2 2 58725E 58729C 58731B 61652C 56.7 3 3 61677A 95 2 2 61700D 70 3 5 198648F 60 3 3 62703D 70784E 73.3 3 3 197496H 73.3 3 3 197496J 73.3 3 3 198649B 80 3 3 56531H 50075F 53301F 60 2 2 51410K 53292D 56410C

TABLE 5B Combining Ability Report for Inbred PH86T F1 data for Inbred Line PH86T when crossed to different tester lines than in Table 5A YIELD YIELD YIELD bu/a 56# bu/a 56# bu/a 56# MST pct MST pct MST pct ABS ABS ABS ABS ABS ABS Tester Mean Locs Reps Mean Locs Reps 56807A 203.1 14 14 18.5 15 15 58725J 178.8 13 17 21.2 13 17 58728E 195 15 15 19.6 15 15 58729J 158.8 14 14 19.7 14 14 61649D 195.3 15 15 18.8 15 15 253240F 187.2 13 16 19.3 13 16 253239E 196 12 15 20 13 16 252631E 172.7 12 16 19.2 12 16 253238G 173.1 12 16 18.7 12 16 70783E 174.2 16 16 20.1 16 16 70784H 189.1 24 24 20.2 25 25 73324C 203.6 15 15 19.4 15 15 76019B 193.6 15 15 18.7 15 15 197496D 167.9 13 17 19.3 13 17 197496F 197.2 15 15 19 15 15 197496K 212.3 13 13 21.1 13 13 197497E 201.8 15 15 18.9 15 15 197497F 208.7 16 16 18.4 16 16 198648E 190.7 18 23 19.8 18 23 198649C 194.5 15 15 19 15 15 208558E 171.2 12 16 18.4 12 16 252673B 181.8 13 16 19.9 13 16 252675B 181.6 13 16 20.5 13 16 252723K 182.2 13 16 22.4 13 16 70783J 197.8 13 13 20.6 13 13 56592D 190 90 100 21.4 90 100 265595D 188.1 13 16 20.2 13 16 EGRWTH EGRWTH EGRWTH ESTCNT ESTCNT ESTCNT score ABS score ABS score ABS count ABS count ABS count ABS Tester Mean Locs Reps Mean Locs Reps 56807A 6 1 1 58725J 5 1 1 54.7 5 10 58728E 4 1 1 58729J 5.8 4 4 44 3 3 61649D 6 1 1 253240F 5 1 1 54.2 5 10 253239E 4 1 1 55.6 5 10 252631E 6 1 1 54.9 5 10 253238G 4 1 1 54.1 5 10 70783E 6.4 5 5 46.3 4 4 70784H 6.2 5 5 46 3 3 73324C 6 1 1 76019B 6 1 1 197496D 4 1 1 54.1 5 10 197496F 5 1 1 197496K 6 1 1 197497E 5 1 1 197497F 5 1 1 198648E 5 1 2 198649C 6 1 1 208558E 5 1 1 53.1 5 10 252673B 6 1 1 56.5 5 10 252675B 5 1 1 54.2 5 10 252723K 5 1 1 54 5 10 70783J 5 1 1 56592D 6.2 14 17 56.6 13 27 265595D 5 1 1 54.4 5 10 GDUSHD GDUSHD GDUSHD GDUSLK GDUSLK GDUSLK GDU ABS GDU ABS GDU ABS GDU ABS GDU ABS GDU ABS Tester Mean Locs Reps Mean Locs Reps 56807A 58725J 58728E 58729J 61649D 253240F 253239E 137 1 1 139 1 1 252631E 253238G 70783E 70784H 73324C 76019B 197496D 197496F 197496K 197497E 197497F 198648E 198649C 208558E 252673B 252675B 252723K 139 1 1 141 1 1 70783J 56592D 145.3 15 21 145.5 10 13 265595D PLTHT cm PLTHT cm PLTHT cm EARHT EARHT EARHT ABS ABS ABS cm ABS cm ABS cm ABS Tester Mean Locs Reps Mean Locs Reps 56807A 355 4 4 153.7 4 4 58725J 350.5 3 4 158.1 3 4 58728E 346.1 4 4 149.9 4 4 58729J 309.9 2 2 134.6 2 2 61649D 349.9 4 4 156.2 4 4 253240F 342.9 3 4 148.6 3 4 253239E 339.7 3 4 140.3 3 4 252631E 344.2 3 4 149.2 3 4 253238G 355.6 3 4 158.8 3 4 70783E 311.8 4 4 142.2 4 4 70784H 325.1 5 5 157.5 5 5 73324C 359.8 3 3 160 3 3 76019B 356.9 4 4 165.7 4 4 197496D 342.9 3 4 155.6 3 4 197496F 343.5 4 4 148 4 4 197496K 335.3 4 4 147.3 4 4 197497E 349.9 4 4 168.3 4 4 197497F 342.3 4 4 163.8 4 4 198648E 320 4 4 152.4 4 4 198649C 354.3 4 4 163.2 4 4 208558E 353.7 3 4 150.5 3 4 252673B 346.1 3 4 148.6 3 4 252675B 350.5 3 4 149.9 3 4 252723K 332.7 3 4 140.3 3 4 70783J 331.9 3 3 152.4 3 3 56592D 327.3 20 25 140.9 20 25 265595D 337.2 3 4 143.5 3 4 STAGRN STAGRN STAGRN TSTWT TSTWT TSTWT score ABS score ABS score ABS lb/bu ABS lb/bu ABS Ib/bu ABS Tester Mean Loos Reps Mean Locs Reps 56807A 6.4 7 7 57.1 11 11 58725J 6.9 6 7 54.1 13 17 58728E 6.3 7 7 57.3 11 11 58729J 4.8 4 4 54.3 13 13 61649D 5.9 7 7 56.3 10 10 253240F 4.7 6 7 54.8 13 16 253239E 5.9 6 7 53.9 13 16 252631E 3.6 6 7 55.8 12 16 253238G 4.3 6 6 56.8 12 16 70783E 6 6 6 56.6 15 15 70784H 6.7 9 9 57.1 23 23 73324C 6.8 6 6 55.3 12 12 76019B 5.3 6 6 57.4 11 11 197496D 3.9 6 7 55.1 13 17 197496F 5.8 6 6 56.6 12 12 197496K 7 6 6 55.8 9 9 197497E 5.8 6 6 54.8 10 10 197497F 6 6 6 55.4 11 11 198648E 6.6 7 9 56 12 15 198649C 6.2 6 6 56.6 11 11 208558E 4.4 6 7 54.6 12 16 252673B 4.7 6 7 55.7 13 16 252675B 5.3 6 7 55.6 13 16 252723K 6.4 6 7 53.5 13 16 70783J 6.7 6 6 55.4 11 11 56592D 6.5 27 31 55.6 80 91 265595D 6.1 6 7 56.4 13 16 ERTLPN ERTLPN ERTLPN GLFSPT GLFSPT GLFSPT % NOT % NOT % NOT score ABS score ABS score ABS ABS ABS ABS Tester Mean Locs Reps Mean Locs Reps 56807A 7 2 2 100 2 2 58725J 6 1 1 86.7 2 3 58728E 7.5 2 2 85 2 2 58729J 61649D 5.5 2 2 95 2 2 253240F 4 1 1 100 1 1 253239E 5 1 1 90 1 1 252631E 5 1 1 93.3 2 3 253238G 4 1 1 90 2 3 70783E 70784H 7 2 2 100 1 1 73324C 7 2 2 56.7 3 3 76019B 7 2 2 63.3 3 3 197496D 4 1 1 90 2 197496F 6 2 2 96.7 3 3 197496K 6.5 2 2 53.3 3 3 197497E 5.5 2 2 53.3 3 3 197497F 6 2 2 36.7 3 3 198648E 50 3 5 198649C 5 2 2 63.3 3 3 208558E 4 1 1 92.5 3 4 252673B 5 1 1 90 1 1 252675B 6 1 1 90 1 1 252723K 5 1 1 100 1 1 70783J 7 2 2 90 3 3 56592D 6.1 6 9 77.5 8 12 265595D 5 1 1 90 1 1 SLFBLT SLFBLT SLFBLT ANTROT ANTROT ANTROT score ABS score ABS score ABS score ABS score ABS score ABS Tester Mean Locs Reps Mean Locs Reps 56807A 4.5 2 2 6 1 1 58725J 58728E 6 2 2 4 1 1 58729J 61649D 5 2 2 4 1 1 253240F 253239E 252631E 253238G 70783E 5 1 1 70784H 6 1 1 4 2 2 73324C 7 2 2 4 2 2 76019B 5 2 2 4.5 2 2 197496D 197496F 5.5 2 2 6 2 2 197496K 5 2 2 4.5 2 2 197497E 6 2 2 3.5 2 2 197497F 6 2 2 4 2 2 198648E 4.5 4 6 2.5 2 2 198649C 6 2 2 4 2 2 208558E 252673B 252675B 252723K 70783J 5 2 2 4 2 2 56592D 5 5 9 4.6 6 11 265595D COMRST COMRST COMRST SOURST SOURST SOURST score ABS score ABS score ABS score ABS score ABS score ABS Tester Mean Locs Reps Mean Locs Reps 56807A 58725J 5.5 1 2 58728E 58729J 5 1 1 61649D 253240F 3 1 2 253239E 4.5 1 2 252631E 2.5 1 2 253238G 3 1 2 70783E 4 1 1 70784H 4 1 1 73324C 76019B 1974960 3.5 1 2 197496F 197496K 197497E 197497F 198648E 198649C 208558E 4 1 2 252673B 3 1 2 252675B 3.5 1 2 252723K 4.5 1 2 70783J 56592D 5 1 1 4 1 2 265595D 5 1 2 STLLPN STLLPN STLLPN EBTSTK EBTSTK EBTSTK % NOT % NOT % NOT % NOT % NOT % NOT ABS ABS ABS ABS ABS ABS Tester Mean Locs Reps Mean Locs Reps 56807A 90.1 3 3 58725J 87.5 2 4 58728E 72.8 3 3 58729J 98.7 2 2 61649D 60.8 3 3 253240F 83.3 3 6 253239E 70 3 6 252631E 55 2 4 253238G 32.5 2 4 70783E 84.4 2 2 97.9 3 3 70784H 84.9 3 3 97.4 2 2 73324C 61.6 3 3 76019B 79.1 3 3 197496D 32.5 2 4 197496F 85.5 3 3 197496K 70 3 3 197497E 57.4 3 3 197497F 82.4 3 3 198648E 54.4 2 2 198649C 66 3 3 208558E 40 3 5 252673B 55 3 6 252675B 73.3 3 6 252723K 66.7 3 6 70783J 65.1 3 3 56592D 75.2 16 34 96.6 3 3 265595D 81.7 3 6

TABLE 6A INBREDS IN HYBRID COMBINATION REPORT Variety #1: HYBRID CONTAINING PH86T - Variety #2: 31G98 YIELD YIELD MST EGRWTH ESTCNT GDUSHD BU/A 56# BU/A 56# PCT SCORE COUNT GDU Stat ABS % MN % MN % MN % MN % MN Mean1 199.5 106.6 101.6 107.4 103.2 101.4 Mean2 191.6 101.9 97.9 92.5 100.4 102.2 Locs 117 117 118 16 15 17 Reps 127 127 128 19 27 23 Diff 7.9 4.7 −3.7 14.9 2.9 −0.8 Prob 0.000 0.000 0.000 0.001 0.191 0.534 GDUSLK STKCNT PLTHT EARHT STAGRN LRTLSC GDU COUNT CM CM SCORE SCORE Stat % MN % MN % MN % MN % MN ABS Mean1 100.7 100.7 102.3 102.1 127.8 5.0 Mean2 101.8 100.4 100.5 103.7 99.6 2.0 Locs 12 197 31 31 34 1 Reps 15 290 38 38 38 1 Diff −1.1 0.4 1.8 −1.6 28.3 3.0 Prob 0.185 0.380 0.072 0.305 0.000 STKLDS STKLDG EBTSTK ABTSTK TSTWT GLFSPT SCORE % NOT % NOT % NOT LB/BU SCORE Stat ABS % MN % MN % MN ABS ABS Mean1 7.0 107.8 102.3 138.9 55.7 6.3 Mean2 7.1 109.4 101.4 62.8 56.2 5.2 Locs 9 1 3 8 80 6 Reps 9 1 3 29 91 9 Diff −0.1 −1.6 0.9 76.1 −0.5 1.1 Prob 0.917 . 0.845 0.001 0.020 0.016 NLFBLT SLFBLT ANTROT CLN MDMCPX FUSERS SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS Mean1 5.8 4.9 4.7 5.0 4.0 7.0 Mean2 5.8 4.1 4.3 4.5 3.0 5.2 Locs 4 6 6 1 2 9 Reps 7 9 10 4 4 16 Diff 0.0 0.8 0.4 0.5 1.0 1.8 Prob 1.000 0.011 0.224 . 0.295 0.002 DIPERS COMRST SOURST ECB1LF ECB2SC HSKCVR SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS Mean1 4.5 5.0 4.0 3.8 4.3 6.7 Mean2 4.0 5.0 3.5 3.7 4.3 5.3 Locs 2 1 1 2 6 16 Reps 4 1 2 6 10 19 Diff 0.5 0.0 0.5 0.2 0.1 1.4 Prob 1.000 . . 0.874 0.842 0.000 HD GIBROT DIPROT BRTSTK SMT ERTLPN LRTLPN SCORE SCORE % NOT % NOT % NOT % NOT Stat ABS ABS ABS ABS ABS ABS Mean1 5.0 4.0 100.0 95.1 82.7 89.2 Mean2 4.5 3.0 98.2 98.8 72.4 85.8 Locs 1 1 1 6 11 12 Reps 2 2 2 9 16 13 Diff 0.5 1.0 1.8 −3.7 10.3 3.3 Prob . . . 0.048 0.145 0.339

TABLE 6B INBREDS IN HYBRID COMBINATION REPORT Variety #1: HYBRID CONTAINING PH86T - Variety #2: 32K61 YIELD YIELD MST EGRWTH ESTCNT GDUSHD GDUSLK STKCNT BU/A 56# BU/A 56# PCT SCORE COUNT GDU GDU COUNT Stat ABS % MN % MN % MN % MN % MN % MN % MN Mean1 199.2 105.8 101.6 105.6 105.6 101.4 100.7 100.7 Mean2 174.6 92.3 92.6 127.2 104.6 102.1 101.0 101.0 Locs 102 102 102 11 7 17 12 165 Reps 112 112 112 14 15 23 14 250 Diff 24.5 13.6 −9.0 −21.6 1.0 −0.6 −0.3 −0.3 Prob 0.000 0.000 0.000 0.032 0.116 0.342 0.570 0.497 PLTHT EARHT STAGRN STKLDG ABTSTK TSTWT GLFSPT NLFBLT CM CM SCORE % NOT % NOT LB/BU SCORE SCORE Stat % MN % MN % MN % MN % MN ABS ABS ABS Mean1 101.5 102.1 122.0 107.8 138.9 55.6 6.3 5.8 Mean2 98.9 96.7 95.7 94.9 113.6 57.8 5.8 5.3 Locs 26 26 28 1 8 67 6 4 Reps 33 33 32 1 29 78 9 7 Diff 2.6 5.3 26.3 12.9 25.3 −2.2 0.4 0.5 Prob 0.022 0.007 0.000 . 0.039 0.000 0.363 0.092 SLFBLT ANTROT CLN MDMCPX FUSERS DIPERS SOURST ECB1LF SCORE SCORE SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 4.9 4.6 5.0 4.0 7.0 4.5 4.0 3.8 Mean2 5.2 4.6 4.5 3.3 4.9 4.0 4.0 3.8 Locs 6 5 1 2 9 2 1 2 Reps 9 10 4 4 16 4 2 6 Diff −0.3 0.0 0.5 0.8 2.1 0.5 0.0 0.0 Prob 0.542 1.000 . 0.500 0.004 0.500 . 1.000 HD ECB2SC HSKCVR GIBROT DIPROT BRTSTK SMT ERTLPN LRTLPN SCORE SCORE SCORE SCORE % NOT % NOT % NOT % NOT Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 4.3 6.8 5.0 4.0 100.0 95.1 83.5 89.2 Mean2 3.9 5.6 8.0 6.0 100.0 95.3 85.5 95.8 Locs 6 14 1 1 1 6 10 12 Reps 10 17 2 2 2 9 14 13 Diff 0.4 1.2 −3.0 −2.0 0.0 −0.2 −2.0 −6.7 Prob 0.590 0.005 . . . 0.863 0.735 0.104

TABLE 6C INBREDS IN HYBRID COMBINATION REPORT Variety #1: HYBRID CONTAINING PH86T - Variety #2: 3223 YIELD YIELD MST EGRWTH ESTCNT GDUSHD GDUSLK STKCNT BU/A 56# BU/A 56# PCT SCORE COUNT GDU GDU COUNT Stat ABS % MN % MN % MN % MN % MN % MN % MN Mean1 201.9 105.2 101.9 109.1 97.2 101.9 100.9 100.2 Mean2 183.9 95.3 92.4 95.6 104.6 104.7 102.2 100.6 Locs 69 69 70 13 4 10 6 114 Reps 71 71 72 16 4 13 6 156 Diff 18.0 9.9 −9.5 13.5 −7.5 −2.8 −1.3 −0.5 Prob 0.000 0.000 0.000 0.002 0.256 0.028 0.224 0.358 PLTHT EARHT STAGRN LRTLSC STKLDS STKLDG EBTSTK ABTSTK CM CM SCORE SCORE SCORE % NOT % NOT % NOT Stat % MN % MN % MN ABS ABS % MN % MN % MN Mean1 104.1 104.1 139.6 5.0 7.0 107.8 102.3 144.9 Mean2 98.2 110.7 72.0 6.0 5.4 73.5 105.2 97.3 Locs 17 17 20 1 9 1 3 5 Reps 22 22 22 1 9 1 3 14 Diff 5.9 −6.6 67.5 −1.0 1.6 34.3 −2.9 47.6 Prob 0.000 0.011 0.000 . 0.133 . 0.437 0.028 TSTWT GLFSPT NLFBLT SLFBLT ANTROT CLN MDMCPX FUSERS LB/BU SCORE SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 55.7 6.3 5.5 5.0 5.0 5.0 4.0 7.1 Mean2 56.3 2.4 6.0 3.3 3.8 4.5 3.8 5.9 Locs 49 4 1 5 4 1 2 5 Reps 52 6 2 7 7 4 4 9 Diff −0.5 3.9 −0.5 1.7 1.3 0.5 0.3 1.2 Prob 0.055 0.010 . 0.005 0.063 . 0.500 0.042 HD COMRST ECB1LF ECB2SC HSKCVR BRTSTK SMT ERTLPN LRTLPN SCORE SCORE SCORE SCORE % NOT % NOT % NOT % NOT Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 5.0 5.0 3.4 6.5 100.0 93.0 87.0 100.0 Mean2 4.0 3.3 4.1 4.5 96.6 97.8 88.0 100.0 Locs 1 1 3 12 1 3 5 2 Reps 1 3 5 13 2 6 7 2 Diff 1.0 1.7 −0.7 2.0 3.4 −4.8 −1.0 0.0 Prob . . 0.184 0.000 . 0.111 0.704 1.000

TABLE 6D INBREDS IN HYBRID COMBINATION REPORT Variety #1: HYBRID CONTAINING PH86T - Variety: 31N27 YIELD YIELD MST EGRWTH ESTCNT GDUSHD GDUSLK STKCNT BU/A 56# BU/A 56# PCT SCORE COUNT GDU GDU COUNT Stat ABS % MN % MN % MN % MN % MN % MN % MN Mean1 198.9 105.9 101.5 105.6 105.4 103.1 102.9 100.9 Mean2 197.4 104.3 100.4 85.8 101.5 100.9 101.3 100.7 Locs 102 102 102 11 11 20 15 178 Reps 112 112 112 13 23 25 17 271 Diff 1.5 1.6 −1.1 19.7 3.9 2.3 1.6 0.2 Prob 0.564 0.298 0.154 0.010 0.020 0.025 0.000 0.608 PLTHT EARHT STAGRN STKLDG ABTSTK TSTWT GLFSPT NLFBLT CM CM SCORE % NOT % NOT LB/BU SCORE SCORE Stat % MN % MN % MN % MN % MN ABS ABS ABS Mean1 101.5 102.1 122.0 107.8 138.9 55.5 6.3 5.8 Mean2 95.3 90.5 112.3 109.4 132.1 57.0 6.0 5.6 Locs 27 27 28 1 8 66 6 4 Reps 34 34 32 1 29 77 9 7 Diff 6.2 11.6 9.8 −1.6 6.9 −1.5 0.3 0.1 Prob 0.000 0.000 0.136 . 0.534 0.000 0.542 0.789 SLFBLT ANTROT CLN MDMCPX FUSERS DIPERS SOURST ECB1LF SCORE SCORE SCORE SCORE SCORE SCORE SCORE SCORE Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 4.9 4.6 5.0 4.0 7.0 4.5 4.0 3.8 Mean2 6.7 4.8 4.0 3.8 6.5 3.5 6.0 3.7 Locs 6 5 1 2 9 2 1 2 Reps 9 10 4 4 16 4 2 6 Diff −1.8 −0.2 1.0 0.3 0.5 1.0 −2.0 0.2 Prob 0.001 0.749 . 0.500 0.160 0.295 . 0.874 HD ECB2SC HSKCVR GIBROT DIPROT BRTSTK SMT ERTLPN LRTLPN SCORE SCORE SCORE SCORE % NOT % NOT % NOT % NOT Stat ABS ABS ABS ABS ABS ABS ABS ABS Mean1 4.3 6.8 5.0 4.0 100.0 95.1 82.7 89.2 Mean2 5.4 6.6 5.5 5.5 100.0 96.3 85.9 93.3 Locs 6 14 1 1 1 6 11 12 Reps 10 17 2 2 2 9 15 13 Diff −1.1 0.2 −0.5 −1.5 0.0 −1.2 −3.2 −4.2 Prob 0.108 0.459 . . . 0.518 0.616 0.318

All publications, patents and patent applications mentioned in the specification are indicative of the level of those skilled in the art to which this invention pertains. All such publications, patents and patent applications are incorporated by reference herein for the purpose cited to the same extent as if each was specifically and individually indicated to be incorporated by reference herein.

The foregoing invention has been described in detail by way of illustration and example for purposes of clarity and understanding. However, it is obvious that certain changes and modifications such as backcross conversions and mutations, somoclonal variants, variant individuals selected from large populations of the plants of the instant inbred and the like may be practiced within the scope of the invention, as limited only by the scope of the appended claims. 

1. A seed of maize inbred line designated PH86T, representative seed of said line having been deposited under ATCC Accession No. PTA-4869.
 2. A maize plant, or a plant part thereof, produced by growing the seed of claim
 1. 3. The maize plant of plant of claim 2 wherein said plant has been detasseled.
 4. A tissue culture of regenerable cells produced from the plant of claim
 2. 5. Protoplasts produced from the tissue culture of claim
 4. 6. The tissue culture of claim 4, wherein cells of the tissue culture are produced from a tissue selected from the group consisting of leaf, pollen, embryo, root, root tip, anther, silk, flower, kernel, ear, cob, husk and stalk.
 7. A maize plant regenerated from the tissue culture of claim 4, said plant having all the morphological and physiological characteristics of maize inbred line PH86T, representative seed of said line having been deposited under ATCC Accession No. PTA-4869.
 8. A method for producing an F1 hybrid maize seed, comprising crossing the plant of claim 2 with a different maize plant and harvesting the resultant F1 hybrid maize seed.
 9. A method of producing a male sterile maize plant comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers male sterility.
 10. A male sterile maize plant produced by the method of claim
 9. 11. A method of producing an herbicide resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers herbicide resistance.
 12. An herbicide resistant maize plant produced by the method of claim
 11. 13. The maize plant of claim 12, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.
 14. A method of producing an insect resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers insect resistance.
 15. An insect resistant maize plant produced by the method of
 14. 16. The maize plant of claim 15, wherein the transgenc encodes a Bacillus thuringiensis endotoxin.
 17. A method of producing a disease resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers disease resistance.
 18. A disease resistant maize plant produced by the method of claim
 17. 19. A method of producing a maize plant with decreased phytate content comprising transforming the maize plant of claim 2 with a transgene encoding phytase.
 20. A maize plant with decreased phytate content produced by the method of claim
 19. 21. A method of producing a maize plant with modified fatty acid metabolism or modified carbohydrate metabolism comprising transforming the maize plant of claim 2 with a transgene encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, levansucrase, alpha-amylase invertase and starch branching enzyme, or encoding an antisense of stearoyl-ACP desaturase.
 22. A maize plant having modified fatty acid metabolism or modified carbohydrate metabolism produced by the method of claim
 21. 23. The maize plant of claim 22 wherein the transgene confers a trait selected from the group consisting of waxy starch and increased amylose starch.
 24. A maize plant, or a part thereof, having all the physiological and morphological characteristics of the inbred line PH86T, representative seed of said line having been deposited under ATCC Accession No. PTA-4869.
 25. A method of introducing a desired trait into maize inbred line PH86T comprising: (a) crossing a PH86T plant grown from PH86T seed, representative seed of which has been deposited under ATCC Accession No. PTA-4869, with a plant of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, disease resistance and waxy starch; (b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants; (c) crossing the selected progeny plants with the PH86T plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PH86T listed in Table 1 to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PH86T listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
 26. A plant produced by the method of claim 25, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PH86T listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
 27. The plant of claim 26 wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.
 28. The plant of claim 26 wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.
 29. The plant of claim 26 wherein the desired trait is male sterility and the trait is conferred by a cytoplasmic nucleic acid molecule that confers male sterility.
 30. A method of modifying fatty acid metabolism, phytic acid metabolism or carbohydrate metabolism in maize inbred line PH86T comprising: (a) crossing a PH86T plant grown from PH186T seed, representative seed of which has been deposited under ATCC Accession No. PTA-4869, with a plant of another maize line that comprise a nucleic acid molecule encoding an enzyme selected from the group consisting of phytase, fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense stearoyl-ACP desaturase; (b) selecting F1 progeny plants that have said nucleic acid molecule to produce selected F1 progeny plants; (c) crossing the selected progeny plants with the PH86T plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have said nucleic acid molecule and physiological and morphological characteristics of maize inbred line PH86T listed in Table 1 to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise said nucleic acid molecule and have all of the physiological and morphological characteristics of maize inbred line PH86T listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
 31. A plant having modified fatty acid metabolism, modified phytic acid metabolism or modified carbohydrate metabolism produced by the method of claim 30, wherein the plant comprises the nucleic acid molecule and has all of the physiological and morphological characteristic of maize inbred line PH86T listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions. 